Agilent InfinityLab LC Series Diode Array Detectors User Manual
59
4
Preparing the Module
Setting up an Analysis
Preparing the Detector
For best performance of the detector
•
Let the lamp warm-up and stabilize for at least one hour (initial turn on of the
module requires a longer time depending on the environment and the
application needs); refer to
•
For high sensitivity measurements, a stable environment is required; refer to
on page 28. Prevent drafts from air condition systems.
•
Setting an appropriate reference wavelength could improve the baseline
behavior.
•
Do not work with removed/open front panels/doors. When the system
includes a G1316 TCC (typically located below the detector) and its front
panel is removed while the TCC is set to high temperatures, the up-streaming
air could influence the stability of the detector baseline.
Preparing the HPLC System
1
Turn on the control software and the monitor.
2
Turn on the modules.
3
Start the control software. The screen should show all modules and the
system status is Not Ready.
4
Turn on the modules that require conditioning:
a
Detector lamp (warm-up for at least 60 min to get a stable baseline).
b
Column compartment (set temperature as required).
c
Pump (purge).
d
Sampler (prepare the standard isocratic sample into a vial).
e
Solvents (fill water and Acetontrile into the solvent bottles).
5
Load the default method.
6
Pump the water/acetonitrile (30/70 %) mobile phase through the column for
10 min for equilibration.
