Agilent InfinityLab LC Series Diode Array Detectors User Manual
145
8
Test Functions and Calibration
Wavelength Calibration
Wavelength Calibration
The detector uses the alpha (656.1 nm) and beta (486 nm) emission lines of the
deuterium lamp for wavelength calibration. The sharp emission lines enable
more accurate calibration than is possible with holmium oxide. When
recalibration is started, the 1 nm slit is moved into the light path automatically
(G7117B). The gain is set to zero.
On completion of the scan, the alpha- and beta-line deviations (in nm) are
displayed. These values indicate how far the detector calibration deviates from
the actual positions of the alpha and beta emission lines. After calibration, the
deviation is zero.
To eliminate effects due to absorbing solvents, install the Max-Light Cartridge
Test Cell before starting the test.
When
The detector is calibrated at the factory, and under normal operating conditions should not require
recalibration. However, it is advisable to recalibrate:
•
after maintenance (flow cell or UV-lamp),
•
after repair of components in the optical unit,
•
after exchange of the optical unit or main board,
•
after significant environmental condition changes (temperature, humidity),
•
at a regular interval, at least once per year (for example, prior to an Operational Qualification/Per
-
formance Verification procedure), and
•
when chromatographic results indicate the detector may require recalibration.
Parts required
#
Description
1
Max-Light Cartridge Test Cell or
1
Max-Light Cartridge Cell
Preparations
•
Detector/lamp must be on for more than 1 hour.
•
When using a Max-Light Cartridge Cell a flow rate of 0.5 mL/min with water is required.
NOTE
If the detector is operated in a lab environment that differs at average from the
final test environment (25 °C) then the detector should be recalibrated for this
temperature.
NOTE
If the detector was repaired (opened covers), the wavelength calibration can be
done 10 minutes after lamp on. A final wavelength calibration should be
repeated after complete warm-up of the detector.
