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SIM processing
After the SIM acquisition is done, save the file as
.czi
before processing. Always start with
automatic processing then if needed fine tune the data with manual processing.
Automatic processing
Go to
Processing
tab
Select
Structured Illumination
from the list
Select
the desired dataset (image) to process
Select the output as
SR-SIM
. Other option is
deconvolution of the dataset
Hit
apply
and wait…