Smart setup (only for the confocal mode)
Smart setup is a quick and easy way to create the settings for fluorophores and channels.
Unexperienced users should start with the smart setup. Please note that smart setup might not
working with the multiphoton laser so multiphoton channels should be setup manually.
•
Select your fluorophores
•
Select between simultaneous or sequential imaging.
Fastest
is simultaneous,
best signal
is sequential imaging (imaging all fluorophores in different tracks).
Start/stop acquisition
Find the focus
in the sample
Auto-adjust
exposure
Real time, fast, low
quality live mode to
find places in the
sample, adjust the
correct sample
intensity and to set
up Z-stacks
Slow live mode to find
places in the sample and
adjust the correct sample
intensity. The quality of
the continuous mode is
taken up from the
settings of “Acquisition
mode” menu
Making an image
(acquisition)
