Acquisition tab, SIM imaging
Here is only the
SIM imaging
showed, for a detailed description of the ZEN Acquisition tab,
please check first the “Acquisition tab, confocal” part.
Grid rotation for SIM
imaging, use
5 rotation
for
the best result
Select
SIM
imaging mode
Selecting the emission
filter for the track (OBS!
Exclude FSet77 HE, as this
filter is for the fluorescent
lamp and eyepiece
Selecting excitation laser
for SIM (Elyra)
Resolution, quality and
cropping of the imaging,
make no change here
Select the desired track
number
When laser selected, the
correct grid is picked up by
the software
Be sure you have selected
the 63x/1.4 objective for
SIM imaging
Add a color to the track
Be sure
Frame
or
Z-stack
is
selected if more tracks exist
Change the laser power to get
the correct intensity (see next
page)
Exposure time for the camera,
usually no need to change it,
try to keep it on 100 ms as a
default
