Acquisition mode
In the acquisition mode the quality of the imaging acquisition can be changed.
Selected objective
Scan mode: spot, line or frame
X-Y resolution:
adjusting the X-Y
resolution. If you are unsure, use
the
optimal
button. For 3D
imaging use 512x512 to save
time/avoid bleaching.
Scanning speed:
go up with the
speed to decrease, go down to
increase the quality. No golden
rule, it is sample dependent, if
unsure try the sample with
different speeds, and use the best
for the real acquisition. Again
COMPROMISE between time and
quality, decide what is more
important.
Averaging:
noise (random pixels)
are removed with averaging
process. Select the number of
image to use for averaging (again
time vs. quality!) and choose
between line and frame
averaging.
Bit depth:
select the grayscale
level for the acquisition. Note that
if you need to modify or re-
contrast the image, more bit
depth is needed, so select 16 bit
imaging. Otherwise 8 bit is usually
enough. This doesn’t affect the
imaging time, only the file size