Axioskop 2 FS
plus
INTRODUCTION
Axioskop 2 FS
MOT
Microscopy in a few steps ...
Carl Zeiss
B 40-076 e 02/01
0-7
Microscopy in a few steps using the example of "Setting of transmitted-light brightfield
according to KÖHLER"
☞
Before starting to use the Axioskop 2 FS
plus
and/or Axioskop 2 FS
MOT
microscopes, make
sure to read the notes on instrument safety and the Description (Chapter 1) and Start-up
(Chapter 2) chapters.
(1)
As described in chapter 2, the microscope is ready for operation and switched on according to
section 3.3.
(2)
Place a high-contrast specimen on the mechanical stage (cover slip pointing upwards).
(3)
Swing in 10x objective in the objective slider and focus on the specimen; if possible, always
move the specimen away from the objective!
(4)
Move condenser – e.g. universal condenser 0.9 – (in brightfield position, front lens switched
in) to the upper stop position via the condenser drive. The height adjustment must be set in
such a way that the specimen is not touched by the condenser.
(5)
Close luminous-field diaphragm until it is visible in the field of view, even if not in focus
(Fig. 0-1/
A
).
(6)
Lower universal condenser 0.9 until the edge of the luminous-field diaphragm appears in
focus (Fig. 0-1/
B
).
(7)
Center luminous-field diaphragm using centering screws on the condenser carrier (Fig. 0-1/
C
)
and open it until its edge just disappears behind the field of view (Fig. 0-1/
D
).
(8)
To set the aperture diaphragm (contrast), remove one eyepiece from the binocular tube and
set the aperture diaphragm to approx. 2/3 of the diameter of the objective exit pupil
(Fig. 0-1/
E
). Optimum contrast setting is dependent on the respective specimen.
(9)
Insert eyepiece again and, if required, refocus on the specimen via fine drive.
(10)
Since the field size and the objective aperture change after each objective change, steps (5) to
(9) must be repeated.
(11)
For objective magnifications between 1.25x and 5x, the front lens 0.9 of the universal
condenser must be swung out and the aperture diaphragm opened entirely.
Fig. 0-1
Diaphragm settings
in transmitted-
light brightfield according to
KÖHLER
Summary of Contents for Axioskop 2 FS plus
Page 1: ...Operating Manual Axioskop 2 FS plus FS MOT Research Microscope...
Page 14: ...INTRODUCTION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 0 14 B 40 076 e 02 01...
Page 110: ...OPERATION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 3 40 B 40 076 e 02 01...
Page 122: ...ANNEX Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT A 2 B 40 076 e 02 01...
