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Zeiss Axioskop 2 FS plus, Manual

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Zeiss Axioskop 2 FS plus Manual Download Page 13

Axioskop 2 FS 

plus

INTRODUCTION

Axioskop 2 FS 

MOT

Overall view of the Axioskop 2 FS 

MOT

Carl Zeiss

B 40-076 e    02/01

0-13

Overall view of the Axioskop 2 FS 

MOT

1

Eyepieces

2

Binocular ergotube

3

Stand

4

Incident-light illuminator

5

Transmitted-light illuminator

6

Key panel

7

Condenser

8

Mechanical stage

9

Objectives

Summary of Contents for Axioskop 2 FS plus

Page 1: ...Operating Manual Axioskop 2 FS plus FS MOT Research Microscope...

Page 2: ...ot covered by an update service Unless expressly authorized forwarding and duplication of this document and the software package and the utilization and communication of its contents are not permitted...

Page 3: ...iew of Axioskop 2 FS plus 0 12 Overall view of Axioskop 2 FS MOT 0 13 CHAPTER 1 INSTRUMENT DESCRIPTION 1 2 1 1 Name and intended application 1 2 1 2 Instrument description and main features 1 2 1 3 Ax...

Page 4: ...ol unit and keypad to the Axioskop 2 FS MOT 2 21 2 1 16 Switch on off the control unit of the Axioskop 2 FS MOT 2 21 2 1 17 Attachment of round cable holder 2 22 2 2 Attachments and conversions 2 23 2...

Page 5: ...3 19 3 3 Illumination and contrasting techniques 3 20 3 3 1 Setting of transmitted light brightfield according to K HLER 3 20 3 3 2 Setting of transmitted light darkfield 3 23 3 3 3 Setting of transm...

Page 6: ...ICE 4 3 4 1 Instrument care 4 3 4 2 Instrument maintenance 4 4 4 2 1 Performing checks 4 4 4 2 2 Changing the fuses 4 4 4 3 Troubleshooting 4 5 4 4 Spares consumables and tools 4 9 4 5 Requesting serv...

Page 7: ...a way that the specimen is not touched by the condenser 5 Close luminous field diaphragm until it is visible in the field of view even if not in focus Fig 0 1 A 6 Lower universal condenser 0 9 until...

Page 8: ...e instruments meet the requirements of the EC directive 89 336 EC and the EMC legislation of November 9th 1992 This operation manual includes information and warnings which must be observed by the use...

Page 9: ...ent will be damaged The power plug must be inserted in a socket featuring a grounding earth contact The grounding effect must not be made ineffective by an extension cable which does not have a protec...

Page 10: ...are used the filter protecting from heat emitted by the microscope illuminator must not be removed since fluorescence filters are sensitive to heat and their performance might therefore be impaired A...

Page 11: ...antee is provided for defects caused by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any othe...

Page 12: ...view of the Axioskop 2 FS plus Axioskop 2 FS MOT 0 12 B 40 076 e 02 01 Overall view of the Axioskop 2 FS plus 1 Eyepieces 2 Binocular tube 3 Stand 4 Incident light illuminator 5 Transmitted light illu...

Page 13: ...view of the Axioskop 2 FS MOT Carl Zeiss B 40 076 e 02 01 0 13 Overall view of the Axioskop 2 FS MOT 1 Eyepieces 2 Binocular ergotube 3 Stand 4 Incident light illuminator 5 Transmitted light illuminat...

Page 14: ...INTRODUCTION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 0 14 B 40 076 e 02 01...

Page 15: ...iption and main features 1 2 1 3 Axioskop 2 FS plus manual microscope configurations and modules 1 4 1 4 Axioskop 2 FS MOT microscope configurations and modules 1 8 1 5 Objectives 1 12 1 6 Eyepieces 1...

Page 16: ...modular design existing microscope modules of the Axioplan 2 such as tubes intermediate tubes and condensers are easy to attach The Axioskop 2 FS plus is offered as a manual and a motorized version Th...

Page 17: ...r 0 9 with swing in front lens for applications in brightfield darkfield phase contrast and differential interference contrast 2 position objective slider with W 0 8 thread ICS Achroplan objectives Bi...

Page 18: ...2 FS plus Carl Zeiss Axioskop 2 FS plus microscope configurations Axioskop 2 FS MOT 1 4 B 40 076 e 02 01 1 3 Axioskop 2 FS plus manual microscope configurations and modules Fig 1 1 Axioskop 2 FS plus...

Page 19: ...lar tube 30 23 4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen lamp 7 Separate 12 V DC 100 W power unit 8 FL filter slider 9 DIC slider 10 Objective 11 Dust cover 12 DIC prism 1...

Page 20: ...inators HAL 12 V 100 W halogen illuminator HBO 103 illuminator Separate 12 V DC 100 W power unit stabilized HBO 100 W transformer for HBO 100 W 2 and HBO 103 W 2 447219 0000 000 487211 9804 458417 000...

Page 21: ...ation H 365 filter set 09 blue excitation 450 490 filter set 15 green excitation H 546 Analyzer module D Optovar module 1 25x Optovar module 1 6x Optovar module 2 5x 452888 0000 000 488001 0000 000 48...

Page 22: ...xioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT microscope configurations Axioskop 2 FS MOT 1 8 B 40 076 e 02 01 1 4 Axioskop 2 FS MOT microscope configurations and modules Fig 1 2 Axioskop 2 FS MOT mi...

Page 23: ...4 HBO 100 W transformer 5 HBO 103 illuminator 6 HAL 12 V 100 W halogen illuminator 7 Separate 12 V DC 100 W power unit 8 FL filter slider 9 DIC slider 10 Objective 11 Control unit 12 Keypad 13 Dust c...

Page 24: ...BO 103 illuminator Separate 12 V DC 100 W power unit stabilized HBO 100 W transformer for HBO 100 W 2 and HBO 103 W 2 447219 0000 000 487211 9804 000 458417 0000 000 458451 9901 000 Objectives see sec...

Page 25: ...xcitation 450 490 filter set 15 green excitation H 546 Analyzer module D Optovar module 1 25x Optovar module 1 6x Optovar module 2 5x 452888 0000 000 488001 0000 000 488009 0000 000 488015 0000 000 45...

Page 26: ...objective Ph1 phase contrast objective with a green color ring and phase stop Ph1 W objectives for water immersion Color ring code for objective magnification Color ring on objective black brown red...

Page 27: ...lastic oiler containing 20 ml of 581 N immersion oil nD 1 515 is particularly suitable for this purpose To prevent oil contamination of the specimen when the nosepiece is turned the resilient mounts o...

Page 28: ...nt image flatness up to the edge of the field of view PL 10x 23 Br foc eyepieces ensure excellent optical correction particularly in combination with the ergotubes If required eyecups for the eyepiece...

Page 29: ...ance 0 001 mm 474066 9901 000 Net micrometer 12 5x12 5 5 10 d 26mm area 12 5x12 5 mm divided in fields of 10x10 474068 0000 000 Photo reticle MC 2 5x d 26 mm for 35 mm photography with an additional m...

Page 30: ...kg Axioskop 2 FS MOT approx 18 kg Ambient conditions Storage and transport in packaging Permissible ambient temperature 10 to 60 C Permissible relative humidity 20 to 85 at 35 C Atmospheric pressure 8...

Page 31: ...VA HBO 100 W transformer HBO 100 W 2 and HBO 103 W 2 Operation closed rooms Protection class I Enclosure protection IP 20 Line voltage can be changed between 115 VAC and 230 VAC Line frequency 50 to 6...

Page 32: ...brightfield insert with turret disk with 5 or 7 positions for brightfield darkfield for phase contrast 1 2 3 for DIC I II III changeable Objective change manually via 2 position slider H DIC W 0 8 FS...

Page 33: ...ol unit and keypad to the Axioskop 2 FS MOT 2 21 2 1 16 Switch on off the control unit of the Axioskop 2 FS MOT 2 21 2 1 17 Attachment of round cable holder 2 22 2 2 Attachments and conversions 2 23 2...

Page 34: ...ransformer 2 18 Fig 2 21 Adjusting aid 2 18 Fig 2 22 Adjustment of mercury vapor short arc lamp 2 18 Fig 2 23 Focal spots of HBO 103 before coarse adjustment 2 19 Fig 2 24 Focal spots of HBO 103 after...

Page 35: ...tube 2 33 Fig 2 47 Attachment of intermediate tube with height adjustment 2 34 Fig 2 48 Attachment of adapter for Axioskop 2 FS plus 2 35 Fig 2 49 Changing the technique module 2 36 Fig 2 50 Changing...

Page 36: ...START UP Axioskop 2 FS plus Carl Zeiss List of illustrations Axioskop 2 FS MOT 2 4 B 40 076 e 02 01...

Page 37: ...ntrol unit and the keypad for the motorized version The following modules are factory attached to the microscope stand stage carrier with mechanical stage universal mounting frame filter mount and SEN...

Page 38: ...W 3 ball headed screwdriver Remove all adhesive tapes including those at the drive knobs Use SW 3 ball headed screwdriver to loosen both fixation screws 2 1 1 of the carrying handle bases 2 1 3 and re...

Page 39: ...sition and tighten the hexagonal screw using the ball headed screwdriver 2 1 4 Remove the stage carrier To screw in the objectives and insert the condenser and if required the DIC slider it is recomme...

Page 40: ...n via the pushrod 2 5 1 also see page 3 8 Remove objective 2 5 3 with the required magnification factor from the case and screw it in the front mount of the objective slider until stop Move rear objec...

Page 41: ...6 2 and stage carrier 2 6 4 Orient the locking screw on the underside of the condenser in the direction of the groove 2 6 7 Press dovetail of condenser against the spring mount 2 6 6 of the condenser...

Page 42: ...n the provided opening 2 8 2 of the objective slider above the objective Make sure that the DIC slider precisely locks into position Bring rear objective mount in the work position see page 3 8 and pr...

Page 43: ...de to engage in the dovetail mount of the stand Slightly tighten the clamping lever 2 9 4 with your right hand Depending on the thickness of the cover slips used align the stage carrier with the top l...

Page 44: ...bes see pp 1 6 1 10 Inserting the eyepiece reticle The eyepieces PL 10x 23 Br foc are intended for use with eyepiece reticles The slight image shift caused by the additional path through the glass is...

Page 45: ...pecimen through the eyepiece set as described above Then use the second focusing eyepiece to focus the microscope image for the second eye The position of the focusing drive on the microscope stand mu...

Page 46: ...2 V DC 100 W power unit and directly switched on off from there Connect plug of halogen illuminator to 12 V 100 W socket 2 14 1 of the separate power unit also see 2 1 13 Should the halogen illuminato...

Page 47: ...mer and switched on off via the power unit of this transformer Insert multi pin plug of the HBO 103 illuminator into the instrument socket 2 16 2 of the HBO 100 W transformer and secure it with captiv...

Page 48: ...AL 100 halogen illuminator a Coarse adjustment Loosen clamping screw 2 17 2 and remove the halogen illuminator from the microscope stand Switch on separate 12 V DC 100 W power unit as described under...

Page 49: ...nd required filters 2 1 14 HBO 103 illuminator 1 Attach HBO 103 W 2 mercury vapor short arc lamp The HBO 103 illuminator and the HBO 103 W 2 mercury vapor short arc lamp are supplied in separate packa...

Page 50: ...se adjustment Remove HBO 103 illuminator from the microscope stand by loosening clamping screw 2 19 2 Switch on HBO 103 on HBO 100 W transformer 2 16 1 and allow it to heat to operating temperature Ma...

Page 51: ...can be performed repeatedly Fine adjustment using adjusting aid Attach HBO 103 illuminator to microscope stand as described in section 2 1 14 2 Pull adjusting aid 2 21 2 out of the incident light tube...

Page 52: ...stand as described in section 2 1 14 2 Remove one objective from the nosepiece and switch empty opening in function position Place a white sheet of paper on the mechanical stage Fully open the precen...

Page 53: ...the control unit and the keypad to the Axioskop 2 FS MOT Connect keypad plug to the Keypad socket 2 27 2 at the rear of the control unit Plug one end of the connecting cable to the Motor Microscope s...

Page 54: ...mechanical stages can be used to attach electrical cables e g from dictating machines For this purpose the round cable holder should be adhered to a suitable position on the microscope The round cabl...

Page 55: ...crew both centering screws 2 30 3 until condenser can be easily taken out of its mount Lift available condenser from the orientation groove by slightly tilting it upwards and pull it out to the front...

Page 56: ...ase stop to be changed in the exchange opening and hold it on the knurled ring since no click stop is effective in this position Use mounting device contained in tool set to unscrew retainer ring 2 31...

Page 57: ...ove DIC prism 2 32 2 from the tool and screw on the new required DIC prism Installation of the DIC prism is made in reverse order Make sure that the DIC prism is oriented correctly groove 2 32 5 must...

Page 58: ...er 2 34 1 to screw stop bolt 2 34 2 into the rear threaded hole of the condenser carrier 2 34 3 until stop 2 2 6 Attachment of SENARMONT DIC polarizer Hold polarizer 2 35 6 parallel to the underside o...

Page 59: ...d in lamp base 2 36 4 let go the spring lever and pull the protection cap off Press spring lever briefly downwards again to center the lamp Attach lamp housing again and tighten clamping screw 2 36 5...

Page 60: ...gloves Use SW 3 ball headed screwdriver to loosen clamping screw 2 38 2 and remove HBO 103 illuminator 2 38 3 from the incident light tube 2 38 1 of the microscope stand Use focusing knob 2 39 3 to br...

Page 61: ...and do not put any pressure on the lamp Correct placement in the heat sink is indicated with H for HBO or X for XBO Clamp fixing screw onto heat sink Place new lamp 2 40 1 with heat sink into the app...

Page 62: ...6 in such a way that the threaded holes 2 41 2 on the underside of the stage are positioned above the openings of the stage carrier 2 41 3 Insert four fixation screws 2 41 5 through the stage carrier...

Page 63: ...ion recentering should be performed as follows Loosen stage clamping screw 2 43 4 and screwing cap of the stage carrier 2 43 1 Rotate the stage to determine the maximum specimen deflection 2 43 5 tip...

Page 64: ...sleeves back on the drive knobs in reverse order and tighten both the clamping screws 5 Setting the smoothness torque of the two drive knobs of the ergo drive The ergo drive is set to a medium smoothn...

Page 65: ...from the mechanical stage 2 45 4 in upward direction Attach required mounting frame or specimen holder 2 45 5 to the mechanical stage and tighten the two countersunk screws 2 2 10 Changing the binocul...

Page 66: ...m the dovetail of the intermediate tube with height adjustment 2 47 5 Insert dovetail of intermediate tube in the stand opening 2 47 8 and tighten clamping screw 2 47 7 only slightly Align intermediat...

Page 67: ...in an upward direction Insert dovetail of the adapter for the Axioskop 2 FS plus 2 48 3 in the stand opening 2 48 6 on the underside and slightly tighten clamping screw 2 48 5 Insert fixation screw 2...

Page 68: ...et Then press the module at the top until it also snaps securely into the top spring clamps of the reflector turret Aligning the respective module when installing it in the reflector turret FL P C ref...

Page 69: ...ce The barrier filter is inserted at 2 50 2 the excitation filter at 2 50 4 and both are secured using retainer ring 2 50 1 2 2 15 Changing the beam splitter in the FL P C reflector module Attachment...

Page 70: ...module half Make sure that the side latch of the spring frame is in the appropriate recess in the bottom module half The reflective side of the beam splitter is recognizable by carefully placing a wo...

Page 71: ...OT 3 19 3 3 Illumination and contrasting techniques 3 20 3 3 1 Setting of transmitted light brightfield according to K HLER 3 20 3 3 2 Setting of transmitted light darkfield 3 23 3 3 3 Setting of tran...

Page 72: ...n the Slow range 3 15 Fig 3 15 Switch on control unit for focusing drive 3 19 Fig 3 16 Microscope settings on the Axioskop 2 FS plus in transmitted light brightfield 3 21 Fig 3 17 Setting the height s...

Page 73: ...focusing directly on the microscope is not possible All the other functions of the manual and the motorized versions are completely identical In the chapter entitled Operation the manual operation fu...

Page 74: ...s Carl Zeiss Operation and function controls Axioskop 2 FS MOT 3 4 B 40 076 e 02 01 3 1 1 Operation and function controls of the Axioskop 2 FS plus manual Fig 3 1 Operation and function controls of th...

Page 75: ...n both sides 11 Focusing drive fine adjustment on both sides 12 Lever to swing in out the diffusion disk 13 Drive for movement of the mechanical stage in the y direction 14 Drive for movement of the m...

Page 76: ...open Pushrod pulled out closed Compartment for filter slider For 3 position or 6 position FL filter slider with 18 mm filter diameter Centering screws for aperture diaphragm incident light Centering o...

Page 77: ...he image brightness in the field of view maximum attenuation factor 1 4400 Lever correspond to on the stand from front to back 1 5 6 and 25 transmission lever in front position filter swung out lever...

Page 78: ...n Turn the pushrod to the right and remove front 3 4 2 or rear 3 4 3 objective from the work position Pull or push the pushrod 3 4 1 and bring front or rear objective into the beam path Turn the pushr...

Page 79: ...intensity Controls the DC supply of the halogen illuminator in the 3 12 V range Display of the existing operating voltage at the scale of the halogen illuminator Binocular ergotube 6 25 23 The binocu...

Page 80: ...yepieces or to the attached camera via a pushrod Pushrod 3 7 2 pushed in 100 of the light for the eyepieces Pushrod 3 7 2 pulled out 100 of the light for the camera To set the most convenient viewing...

Page 81: ...0 when used individually or in combination When several filters are combined the transmission value is calculated as the product of the individual transmission values in percent Filter slider The 3 po...

Page 82: ...ased continuously from 60 to 110 mm This intermediate tube can only be used in combination with the binocular ergotube 6 25 23 or the binocular ergo phototube 6 25 23 100 100 Loosen clamping lever 3 1...

Page 83: ...ion and function controls Carl Zeiss B 40 076 e 02 01 3 13 3 1 2 Keypad for objective focusing of the Axioskop 2 FS MOT single hand control 1 Keypad 2 Display 3 Handwheel for fine adjustment of the fo...

Page 84: ...Select fast focusing speed Select slow focusing speed Activate stored focus position 1 Activate stored focus position 2 Activate stored focus position 3 Activate stored focus position 4 Input key for...

Page 85: ...ward movement of the focusing drive When one of the direction keys is pressed the setting of the focusing drive is changed at the preset speed until the key is released again The speed of the focusing...

Page 86: ...or the first time the current position value of this key is simply overwritten a Store the focus position e g Pos 2 Set the required focus position via the direction keys or the handwheel First press...

Page 87: ...3 18 If the objective slider is in the Home position the other keys of the keypad are not active Set step width and perform steps The Step function allows the focusing drive to be reproducibly shifted...

Page 88: ...e slider and activated or deactivated any time If the end switches for the work range are activated the Home function is not available a Set end switches Use the direction key or the handwheel to appr...

Page 89: ...light illumination via the on off switch 3 1 23 on the instrument front Use the light intensity control 3 1 24 to set the required brightness The scale on the control 3 1 24 indicates the existing lam...

Page 90: ...S plus 2 Transmitted light brightfield equipment of the manual Axioskop 2 FS plus Each manual Axioskop 2 FS plus configuration permits performance of the transmitted light brightfield technique 3 Tran...

Page 91: ...iece from the tube and look into the tube with your naked eye Use sliding knob 3 16 4 to set the aperture diaphragm to approx 2 3 4 5 of the diameter of the objective exit pupils 3 16 E In most applic...

Page 92: ...guration of the Axioskop 2 FS MOT Each Axioskop 2 FS MOT configuration permits the performance of the transmitted light brightfield technique 6 Setting the Axioskop 2 FS MOT for transmitted light brig...

Page 93: ...ure of 0 75 Objectives with a higher aperture can only be used together with the above universal condenser if they feature an integrated aperture iris stop 3 Transmitted light darkfield settings on th...

Page 94: ...d and the luminous field diaphragm image will be almost perfectly in focus Finally match the diameter of the luminous field diaphragm to the size of the field of view Darkfield microscopy requires spe...

Page 95: ...phase ring and a constant phase shift is applied The indirect light components diffracted at different cell components however by pass this optical channel and are influenced by the in phase refracti...

Page 96: ...ect 3 20 A the two SW 1 5 screwdrivers 3 19 1 and 4 must be used on the two centering screws 3 19 2 and 3 to recenter the bright phase stop until complete congruence with the dark phase ring is achiev...

Page 97: ...ives used are integrated in the objective slider and centered Condenser with turret disk with DIC prisms DIC I DIC II DIC III Polarizer e g SENARMONT polarizer In the case of the achromatic aplanatic...

Page 98: ...le objective in the objective slider in the work position via the pushrod 3 22 2 Swing in the DIC analyzer module on the reflector turret 3 22 1 Swing in the suitable DIC prism I II or III digit on co...

Page 99: ...are positioned between polarizer and analyzer The same applies to Plexiglas culture chambers if the chamber is made of plastic In such cases it is recommended to use chambers with glass bottoms to av...

Page 100: ...the relevant dichroic beam splitter must be perfectly matched 2 Axioskop 2 FS plus and Axioskop 2 FS MOT configuration FL P C reflector module and shutter plate in reflector turret HBO 103 or HBO 50 m...

Page 101: ...objective exit pupil is unblocked Insert eyepiece in tube again and close luminous field diaphragm 3 23 5 until it is visible in the field of view Use the two centering screws 3 23 6 to center the lu...

Page 102: ...ography equipment please see the relevant operating instructions 1 Attachment of a SLR camera e g CONTAX 167 MT Screw T2 adapter 416010 0000 000 3 24 3 suitable for the used camera on the 2 5x connect...

Page 103: ...e adapter 456006 0000 000 3 25 5 in phototube 3 25 6 and tighten three hexagonal screws 3 25 7 Insert P 2 5x projection lens 456021 0000 000 3 25 4 in the microscope camera adapter 3 25 5 Attach MC 80...

Page 104: ...cameras T2 Adapter for SLR cameras Cat No T2 Adapter for CONTAX CONTAX bayonet 416010 0000 000 T2 Adapter for OLYMPUS OM OM bayonet 416002 0000 000 T2 Adapter for MINOLTA SR bayonet 416003 0000 000 T2...

Page 105: ...as to the phototube of the Axioskop 2 FS plus Axioskop 2 FS MOT Tube Adapters Cameras 456105 456105 0000 000 60 C 2 3 1 0 456107 000000 1069 414 60 C 2 3 0 63 456106 456106 0000 000 60 C 1 2 0 5 45610...

Page 106: ...40 456140 0000 000 Adapter 60 44 Microscopes with 60 mm interface and all video adapters for 44 mm interface Connects video adapters for 44 mm interface to microscopes with 60 mm interface 3 4 3 Attac...

Page 107: ...his scale value is exactly that distance in the specimen which complies to one interval of the used crossline micrometer For calibration align the scales of the stage micrometer and the crossline micr...

Page 108: ...s using the microscope are always possible to be performed if both the lower and upper side of the specimen can be focused This should preferably be performed using a precision focusing drive and a hi...

Page 109: ...hat d 9 5 m with an interval of 1 m To make things easier the Axioskop 2 FS MOT permits one focus position to be set to zero and the difference d of the height adjustment of the objective slider to be...

Page 110: ...OPERATION Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 3 40 B 40 076 e 02 01...

Page 111: ...D SERVICE Contents 4 CARE MAINTENANCE TROUBLESHOOTING AND SERVICE 4 3 4 1 Instrument care 4 3 4 2 Instrument maintenance 4 4 4 2 1 Performing checks 4 4 4 2 2 Changing the fuses 4 4 4 3 Troubleshootin...

Page 112: ...CARE MAINTENANCE TROUBLESHOOTING Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT 4 2 B 40 076 e 02 01...

Page 113: ...cs cleaning solution consisting of 15 isopropanol and 85 medical alcohol benzoline clean glass surface using a slightly moistened wad of cotton by moving in circles starting in the middle Remove dust...

Page 114: ...2 Changing the fuses Always pull the power plug before changing the fuses The fuse compartment is positioned at the rear of the relevant power supply unit It is combined with the instrument socket and...

Page 115: ...n the phototube in the correct position end position see page 3 10 Objective in objective slider not lowered to work position Lower objective in objective slider to work position Condenser not set cor...

Page 116: ...f no or unspecified immersion oil with immersion objectives Use immersion oil 518 N from Carl Zeiss see p 4 9 Air bubbles in the immersion oil Apply new oil to remove the bubbles Immersion oil at the...

Page 117: ...ropia s p 2 13 Eye fatiguing microscopy Interpupillary distance of the binocular tube is not set correctly Set interpupillary distance correctly see p 2 13 Focusing eyepieces are not set correctly Set...

Page 118: ...p 2 27 12 V 100 W halogen lamp defective Replace 12 V 100 W halogen lamp see p 2 27 The specified 12 V 100 W halogen lamp is not used Use the specified 6 V 12 W halogen lamp see p 1 6 Fuses are defect...

Page 119: ...yepiece eyecup 444801 0000 000 recommended for low brightness techniques to suppress reflected light Dust cover for eyepiece tube 000000 0168 373 to close instrument openings which are not used 518 N...

Page 120: ...g drive may only be performed by Carl Zeiss service staff or specially authorized personnel To ensure the optimum setting and trouble free function of your microscope even for a longer period of time...

Page 121: ...2 FS plus ANNEX Axioskop 2 FS MOT Overview Carl Zeiss B 40 076 e 02 01 A 1 ANNEX List of abbreviations A 3 List of key words A 5 Certification in accordance with DIN ISO 9001 EN 46001 EC conformity de...

Page 122: ...ANNEX Axioskop 2 FS plus Carl Zeiss Axioskop 2 FS MOT A 2 B 40 076 e 02 01...

Page 123: ...ocus difference in height measurement DC Direct Current DIC Differential Interference Contrast DIN Deutsches Institut f r Normung German standards association DL Transmitted light DX Coding system for...

Page 124: ...Refractive index of D line sodium nP Refractive index of the specimen Ph Phase contrast PL Flatfield R Right drive on the right of the mechanical stage SLR Single Lens Reflex SW Wrench opening T Slow...

Page 125: ...2 22 Automatic mode 3 19 B Basic settings 3 19 Binocular tube 2 7 3 6 Binocular ergo phototube 3 10 Binocular ergotube 3 9 Brightfield 3 7 3 20 3 23 3 25 C Care 4 3 Carrying handle 2 6 Changing the f...

Page 126: ...Fluorescence 3 30 Focusing drive 3 5 3 6 3 22 Front lens 2 9 2 23 3 7 3 20 3 22 Function keys of the keypad 3 14 H Halogen illuminator 2 16 3 9 Handwheel for fine adjustment 3 15 HBO 103 illuminator 2...

Page 127: ...3 7 3 8 Mercury vapor short arc lamp 2 18 3 31 Microscope camera 3 33 Modules 1 4 1 8 O Objectives 2 5 2 31 Objective change 3 5 3 8 Objective slider 1 18 3 21 On off switch 3 9 Operation 1 16 Operat...

Page 128: ...rt up 2 1 2 5 Store the focus position 3 16 Switch illumination on off 2 14 Switching on 3 19 Switch on off 2 14 Switch on off control unit 2 21 T Technical data 1 16 Technique module 2 5 3 8 Tools 4...

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