Zeiss Axio Scope.A1, Operating Manual

Page: 84 / 123

OPERATION
Carl Zeiss Illumination and Contrasting Method Axio Scope.A1
84
M60-2-0007 e 05/08
•
Adjust the knurled knob on the field diaphragm (
663H663H
Fig. 4-11/ 4 ) so that the field diaphragm becomes
visible in the field of view (
664H664H
Fig. 4-11/ A ).
•
Readjust the focus on the edge of the field diaphragm with the focusing drive (
665H665H
Fig. 4-11/ B ) and center
the field diaphragm on the edge of the field of view (
666H666H
Fig. 4-11/ C ) with the centering screws (
667H667H
Fig.
4-11/ 3 ).
•
Now open the field diaphragm enough to just make it disappear behind the edge of the field of view
(
668H668H
Fig. 4-11/ D ).
•
For adjusting the aperture diaphragm (image contrast) take out an eyepiece from the tube barrel and
look into the barrel or mount an auxiliary microscope instead of the eyepiece.
•
Center the aperture diaphragm with the centering screws (
669H669H
Fig. 4-11/ 1 ) and adjust the knurled knob
(
670H670H
Fig. 4-11/ 2 ) to about 2/3 to 4/5 of the diameter of the objective exit pupil (
671H671H
Fig. 4-11/ E ) for samples
with a medium contrast quality.
In most cases this position of the aperture diaphragm gives the best contrast at almost full resolution
which is best compromise for the human eye.
•
Replace the eyepiece and adjust the focus of the coaxial rough and fine drive and the image
brightness according to the reflected light sample.
Never use the aperture diaphragm to regulate the image brightness! Use the control knob on
the auxiliary power supply unit to adjust the light intensity!