Zeiss Axio Scope.A1, Operating Manual, Page: 75 / 123

OPERATION
Axio Scope.A1 Illumination and Contrasting Method Carl Zeiss
M60-2-0007 e 05/08
75
•
If the overlap is not exact (
632H632H
Fig. 4-5/ A ), the
lighter annular diaphragm must be recentered
with the aid of two hexagon screwdrivers
SW 1.5 (
633H633H
Fig. 4-4/ 1 and 4 ). Adjust the two
centering screws (
634H634H
Fig. 4-4/ 2 and 3 ) so that you
achieve a full overlap with the darker phase ring
(
635H635H
Fig. 4-5/ B ).
•
Remove the auxiliary microscope from the tube
and replace it with the eyepiece.
In order to increase the image contrast, an
interference broadband filter, green 32 x 4, may be
mounted on the field diaphragm or inserted into
the color glass carrier (if available).
A complete phase contrast can only be achieved
when the light-colored annular diaphragm (in the
condenser) overlaps exactly with the dark-colored
phase ring (in the object) in the illumination beam
path (
636H636H
Fig. 4-5/ B ).
Fig. 4-5 Centering the annular phase
diaphragm (light-colored, in the
condenser) and the phase ring
(dark-colored, in the object)