Waters Micromass ZQ 2000, Operator'S Manual, Page: 56 / 133

Setting Calibration Parameters 56
4
3. Enter these suggested initial reference solution values in Tune window’s Peak
Editor.
Note: These settings are offered as reference points only and, once adopted, might
require adjusting.
4. Select rows 1 to 4 in the Peak Editor. This specifies four mass peaks in the Tune
window’s Peak Display area (see
Figure 3-3 ).
5. Click the ES+ Source tab, and enter these suggested parameters in the
corresponding fields of the ES+ Source page ( Figure 4-3 ).
Row Mass (ZQ 2000) Mass (ZQ 4000) Span Gain
1 172.9 172.9 5 8
2 772.5 1521.9 5 20
3 1521.9 2271.4 5 40
4 1971.6 3470.5 5 39
Parameter Suggested Value
Capillary (kV) 3.5
Cone (V) 50
Extractor (V) 3
RF Lens (V) 0.5
Source Temperature (
o
C) 80
Desolvation Gas Flow Rate (L/hr) 250
Desolvation Temperature (
o
C) 120
Cone Gas Flow (L/hr) 50
Caution: Failure to flow desolvation gas during ESI operation can damage
the source.