Waters Micromass ZQ 2000, Operator'S Manual

Page: 43 / 133

Tuning in ESI Mode 43
3
Figure 3-3 Tune Window Displaying the ES+ Source Page
3.2 Tuning in ESI Mode
Table 3-1 describes the Tune window’s ES+ Source page parameters.
Note: The voltage parameters shown in this table optimize sensitivity and stability. The
temperature and flow rate parameters control the extent of solvent evaporation and adduct
formation.
Table 3-1 ES+ Source Page Parameters
Parameter Description
Capillary voltage Enhances or suppresses ion density by supplying excess charge
to droplets. Optimal voltages: 2 to 4 kV for positive ions and 2 to
3 kV for negative ions.
Cone voltage Helps draw ions into the first vacuum region (20 to 70 V optimal).
Extractor voltage Focuses ions toward the hexapole RF lens (3 to 10 V optimal).
Increasing voltage can induce fragmentation.
RF Lens voltage Focuses ions toward the center of the quadrupole (~0.5 V).