Waters ACQUITY UPLC H-Class Series, System Manual, Page: 10 / 92

1.3.2 Sample manager-flow through needle (SM-FTN)................................................................ 19
1.3.3 Column heater (CH-A) .........................................................................................................20
1.3.4 Column manager (optional) .................................................................................................20
1.3.5 Column Module Switch Box (optional).................................................................................21
1.3.6 Sample organizer (optional).................................................................................................21
1.3.7 FlexCart (optional) ...............................................................................................................22
1.3.8 Post-Injection Volume Kit (optional).....................................................................................22
1.3.9 Detection..............................................................................................................................22
1.3.10 Column technology ............................................................................................................22
1.4 For additional information..............................................................................................................23
2 Performance optimization........................................................................................25
2.1 General guidelines ........................................................................................................................25
2.1.1 Follow these general recommendations when performing a UPLC analysis....................... 26
2.2 Dispersion .....................................................................................................................................27
2.3 Carryover ......................................................................................................................................27
2.4 Cycle time (between injections) ....................................................................................................28
2.5 Preventing leaks............................................................................................................................28
2.5.1 Installation recommendations for fittings .............................................................................29
2.6 Developing methods .....................................................................................................................42
2.7 Sample preparation.......................................................................................................................42
2.7.1 Particulates ..........................................................................................................................42
2.7.2 Matching sample diluents ....................................................................................................42
2.8 Transferring methods ....................................................................................................................43
2.8.1 Columns calculator .............................................................................................................45
2.8.2 Transferring from HPLC to UPLC ........................................................................................45
2.8.3 Transferring from UPLC to HPLC ........................................................................................45
2.9 Solvent recommendations.............................................................................................................46
3 System preparation ..................................................................................................47
3.1 Powering-on the system................................................................................................................47
3.2 Opening the console .....................................................................................................................48
3.2.1 To open the console from Empower software ..................................................................... 48
3.2.2 To open the console from MassLynx software .................................................................... 48
April 4, 2018, 715005702 Rev. A
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