4.
On the
Equilibrate to Method
tab, click each module sub-tab and if necessary, change
the settings for the flow rate, solvent composition, temperature, and lamp state to match
your requirements at equilibration.
Table 3–14: Equilibrate to Method table values
System startup
parameters
Default
Allowed values
Method initial flow rate 0.500 mL/min
0.1 to 2.0 mL/min
Composition of A, B,
C, and D (sum must
be 100%)
A, 100%
B, C, D, 0%
A; 0 to 100%
B; 0 to 100%
C; 0 to 100%
D; 0 to 100%
Column temperature
Off
Note:
Column
selection for the
Column Manager
defaults to Column
1.
Depends on type of column compartment
Sample temperature
On
Off, or 4.0 to 40.0 °C
Lamp
On
On or Off
Note:
For light guiding flow cells, do not
power-on, operate, or ignite the lamp of the
detector when there is no flow through the
cell, or when the cell is dry.
Note:
Change the settings on the
Optional Characterize
tab only if the needle has been
replaced.
5.
Click
Start
.
Result:
1.
The system begins to prime.
2.
The system establishes the method flow rate, column and sample temperatures, and
ignites the lamp.
3.
After priming, the sample manager characterizes the needle and seal, if selected,
and then logs the results of the characterizations into the database.
April 4, 2018, 715005702 Rev. A
Page 66