Notice:
• Do not leave buffers stored in the system.
• Flush all flow paths, including the needle wash, with plenty of non-buffered solvent
before shutting down the system.
• For extended shutdown periods (longer than 24 hours), use 10% to 20% methanol
in water.
• When using a buffered wash solvent, prime it for a minimum of 30 sec.
• Use of buffers can cause salt build-up on the needle and wash port, which can
require periodic cleaning.
To start up the system:
1.
From the system view of the console, click
Control
>
Start up system
.
Alternative:
Right-click in the control panel and click
Start up system
.
2.
On the
Prime Solvents
tab, click the solvent manager sub-tab and if necessary, change
the value in the
Duration of Prime
field.
Notes:
• If you are priming with solvents that differ significantly from the current solvents, prime
the solvent manager for minimum of 5 minutes.
• If you are using a concentrated acid and a concentrated base, it is recommended that
the lines are not primed in succession. Instead, to reduce the risk of salt formation,
ensure the pump is flushed or primed with water prior to priming the second buffer.
• If you want to return settings to their original values on any tab, click
Set Defaults
.
3.
On the
Prime Solvents
tab, click the sample manager sub-tab and if necessary, change
the settings for the wash and purge solvent.
Table 3–13: Sample manager priming parameter values
Parameter
Wash solvent
Purge solvent
Priming range
1 to 600
seconds
1 to 100 cycles
Note:
Each cycle takes approximately 0.5
minutes.
Default priming
15 seconds
5 cycles
Recommended priming:
dry inlet tube
180 seconds
100-µL syringe: 60 cycles
250-µL syringe: 24 cycles
500-µL syringe: 12 cycles
Recommended priming:
changing solvents
180 seconds
100-µL syringe: 50 cycles
250-µL syringe: 20 cycles
500-µL syringe: 10 cycles
April 4, 2018, 715005702 Rev. A
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