2.8 Transferring methods
Transferring an LC method from one system to another can sometimes be necessary. For such
transfers, the object is to maintain a separation’s performance or enhance it by reducing runtime
or improving selectivity and/or resolution. The ACQUITY UPLC H-Class Series system, which
Waters designed specifically to facilitate method transfer, is the ideal tool to achieve that goal with
quaternary solvent capability, low pressure mixing, and flow through needle design. The optional
column manager facilitates switching between target columns while exhibiting low bandspread
and maintaining the same temperature profile as the standard column heater.
The following example of a method transfer shows the related substances test for galantamine,
an alkaloid used to treat Alzheimer’s disease. The USP method (monograph: USP32-NF27
supplement: no. 2, page 4245) is demonstrated first using an HPLC system. The method is then
transferred to an ACQUITY UPLC H-Class Series system fitted with an HPLC column, whereby
selectivity and resolution are maintained. Using the ACQUITY UPLC columns calculator, it is then
scaled to use a UPLC column and optimized for the shortest analysis time at equal peak capacity.
The run time decreased by 46 minutes.
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