Waters 1500-Series, Operator'S Manual

Page: 105 / 188

Preparing for MassLynx operation 4-21
Before purging
• Make sure the eluent is filtered and degassed.
• Prime and purge the pump with fresh eluent, as described in the section
above.
• Ensure the pump’s reference valve knob is turned to the left, directing
the eluent flow to the system (for the 1525µ pump, position the vent
valve knob to the system).
When you change between two eluents that are not totally miscible, perform
the steps in this procedure with an intermediate eluent before purging with
the final eluent. See Appendix C for more information.
Purging
To purge the flow path:
1. Determine flow rate and gradient settings appropriate for your column
and application, and then follow step 1 through step 6 on page 4-19 .
2. Allow the pump to run for a few minutes to complete the purge.
Tip:
The amount of running time required to purge the flow path
depends on total system volume, eluent miscibility, flow rate, and so on.
3. Verify that the flow exiting to waste is normal and consistent for the
specified flow rate, and then click the Start/Stop Pumping icon in
the Inlet Method window to stop the flow.
For more information about selecting an purge method, refer to the MassLynx
online Help.
Caution:
To avoid damaging the column, ensure any changes to
conditions that affect the column, such as flow or pressure, are gradual.