Waters 1500-Series, Operator'S Manual

Page: 98 / 188

4-14 Preparing for Operation
Before equilibrating
• Make sure the eluent is filtered and degassed.
• Allow the eluent and the system to reach proper temperatures before
pump operation.
• Prime the pump, as described in the section above.
• Ensure the pump’s reference valve knob is turned to the left, directing
the eluent flow to the system (for the 1525µ pump, position the vent
valve knob to the system).
To equilibrate the system:
1. Determine flow rate and gradient settings appropriate for your column
and application, and then follow step 1 through step 4 on page 4-11 .
2. Allow the system to equilibrate for several minutes.
Tip:
The amount of running time required for equilibration depends on
total system volume, eluent miscibility, flow rate, and so on.
3. Monitor the system pressure to verify it is normal and stable.
4. Inspect the eluent flow exiting the system to verify it is normal and
consistent for the specified flow rate.
Tip:
To monitor the detector baseline during system equilibration, in the
Acquisition bar, click the Equilibrate System/Monitor Baseline icon
. Select an instrument method for the pump and click
Equilibrate/Monitor. For information about creating an instrument
method used for equilibration, refer to the Breeze 2 online Help.
5. Click the Stop Flow icon in the Run Samples window to stop the
flow.