Waters 1500-Series, Operator'S Manual

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Connecting pump inlet and outlet lines 2-13
5. Firmly seat the tubing end into the inlet manifold on the pump, and
then finger-tighten the compression screw.
6. If you have a binary pump, repeat step 1 through step 5 for the second
pump assembly.
Preparing solvent reservoirs
General recommendations:
• When using the instrument for general chromatography (that is, reverse
and/or normal phase and gel permeation), use high-quality lab
glassware made of borosilicate glass for all reservoirs (solvent, seal
wash, and needle wash).
• When using techniques such as ion chromatography, where glass
containers can contribute ionic contamination (sodium and/or chloride
ions), use laboratory-grade polypropylene or polyethylene containers as
reservoirs.
• When using the instrument in combination with a mass spectrometer,
refer to recommendations published in the most recent version of
“Controlling Contamination in UPLC/MS and HPLC/MS Systems” on
the Waters Web site.
• Choose 1-L solvent reservoirs that provide a snug fit for the reservoir
caps supplied in the startup kit. The solvent reservoir caps help control
solvent evaporation. Three feather-edged holes in each cap make a gas
tight seal around the solvent and vent tubes.
Place the reservoirs in the solvent bottle tray, and set the tray above the
solvent management system components.
Connecting to an eluent reservoir
Tip:
To avoid having eluent leak from a pump outlet, position each eluent
reservoir at a level below its corresponding pump inlet until the outlet is
connected to the system.
Caution:
To avoid damaging the ferrule, do not overtighten the
compression screw.