Waters 1500-Series, Operator'S Manual

Page: 96 / 188

4-12 Preparing for Operation
3. In the Run Samples window, click the Start Flow icon , and enter
appropriate values for the pump’s flow rate and gradient settings.
Tip:
To quickly prime and purge just the pump,
• for the 1515 pump, set the rate to 2.0 mL/min.
• for the 1525 pump, set the rate to 4.0 mL/min and gradient to 50%
A, 50% B.
• for the 1525µ pump
,
set the rate to 2.0 mL/min and gradient to 50%
A, 50% B.
• for the 1525EF pump, set the rate to 6.0 mL/min and gradient to
50% A, 50% B.
4. Select Immediate Flow Change and click OK.
5. Allow the pump to run for approximately one minute, and then verify
that the eluent flow exiting to waste is normal and consistent for the
specified flow rate.
6. Click the Stop Flow icon in the Run Samples window to stop the
flow.
Purging the flow path via Breeze 2 control
Purging ensures that all eluent in the flow path is replaced with new eluent
before you equilibrate the system and run samples (see page 4-2 ).
Tip:
When bringing new instruments online or troubleshooting a solvent
problem, you can restrict the flow path to only those sections of the system
that require purging by disconnecting the flow path at an inlet or outlet
junction, such as the injector outlet or column outlet, and routing the flow to
waste.