Waters 1500-Series, Operator'S Manual

Page: 103 / 188

Preparing for MassLynx operation 4-19
Preparing for MassLynx operation
Use this section to ready the 1500-series pump for performing runs under
MassLynx software control. It describes simple techniques for accomplishing
these tasks:
• Priming and purging the pump
• Purging the system
• Equilibrating the system
For additional information and instructions on setting up the HPLC system
and creating inlet methods, see the MassLynx online Help.
For software-release-specific details, see the release notes for the Waters
1500-series pumps and the Waters MassLynx software.
Power-on the pump and other chromatographic system devices before you
power-on the MassLynx system workstation, and start the MassLynx
application (see page 4-2 ).
Priming and purging the pump via MassLynx control
You must prime and purge the pump to ensure its proper operation (see
page 4-2 ).
Before priming and purging the pump
• If the pump is dry, dry prime it before proceeding (see page 4-4 ).
• Ensure the eluent flow is directed to waste—bypassing any connected
components downstream of the pump—by turning the pump’s reference
valve knob 1/2-turn to the right (for the 1525µ pump, position the vent
valve knob to Vent).
To prime and purge the pump:
1. At the MassLynx workstation, log on to MassLynx.
2. From the MassLynx Main window, click Status.
3. Click the Inlet Method button to open the Inlet Editor window.
4. Click the Inlet icon to go to the pump method.