Waters 1500-Series, Operator'S Manual

Page: 88 / 188

4-4 Preparing for Operation
• Dedicate an eluent supply tube and filter assembly for each eluent. If
doing so is not possible, purge the tube and filter of any remaining
eluent before you use a new eluent.
• To prevent cross contamination of eluents, dedicate a set of glassware
for preparing, storing and supplying each eluent. Once a container has
been used for an eluent, do not use it for any other eluent unless it has
been thoroughly cleaned.
Dry priming the pump
Dry priming is necessary during initial pump startup or when the entire
eluent line is dry.
Tip:
For binary pumps, you can possibly need to dry prime one or both pump
assemblies.
If your pump configuration includes a plunger seal wash system, you can
possibly need to prime the seal wash pump as well (see page 4-9 ).
Required material
Priming syringe (P/N WAT010337 in startup kit)
To dry prime the pump:
1. Fully depress the priming syringe plunger to remove all air from the
syringe.
Caution:
To avoid chemical hazards, observe safe laboratory practices
when you handle eluents. Refer to the Material Safety Data Sheets for
the eluents you use. For more information on eluent properties, see
Appendix C .