Varian ProStar 325 UV-Vis, Service Manual

Page: 67 / 149

ProStar325 Service Manual
Publication date 8 May 2003
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diluting one part of concentrated phosphoric acid with three
parts of HPLC grade water.
3. After pumping 50ml of the 25% acid through the cell at 1-2
ml/min, rinse by pumping 100 to 200ml of HPLC grade water
through the system. Do not allow the acid to remain in the
cell for more than one hour. Dispose of the acid in accordance
with approved waste disposal procedures.
4. Do a final rinse with acetonitrile or methanol.
The frequency for cell cleaning depends upon the cleanliness
maintained during routine operation.
When making or breaking solvent line fittings, care should be
taken to prevent any contamination of exposed fittings. Tubing
should be sealed or capped when not actually connected. Thread
lubricants should never be applied to compression fittings.
The external windows of the flowcell can be cleaned with a dry,
clean source of compressed air or nitrogen. Remove the flowcell
and carefully blow any dust or contamination from the external
windows. Replace the flowcell, ensuring that the sample and
reference beam windows are not obstructed.
3.21.4 Flowcell types
There are four flowcell varieties available for the ProStar325
detector. Each one has an inlet and outlet connection, quartz
optics cell window and each type is easily identifiable.
9x1 Prep 9x0 Analytical
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