Daily Operation
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_______________________________________________ Things to Do After Operating the LTQ
_______________________ Finnigan LTQ Hardware Manual ______________________
3-7
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d. Set the auxiliary gas flow rate to 5:
In the APCI Source dialog box, enter
5
in the Aux Gas Flow Rate text
box.
e.
Set the sweep gas flow rate to 0:
In the APCI Source dialog box, enter
0
in the Sweep Gas Flow Rate
text box.
f.
Set the APCI spray current to 0:
In the APCI Source dialog box, enter
0
in the Spray Current text box.
g. Click on
OK
.
Go to step 7.
6. Set up the ESI source as follows:
a.
In the LTQ Tune Plus window, choose
Setup > ESI Source
to (or
click on the ESI source button) to display the ESI Source dialog box.
b. Set the sheath gas flow rate to 30:
In the ESI Source dialog box, enter
30
in the Sheath Gas Flow Rate
text box.
c.
Set the auxiliary gas flow rate to 5:
In the ESI Source dialog box, enter
5
in the Aux Gas Flow Rate text
box.
d. Set the sweep gas flow rate to 0:
In the ESI Source dialog box, enter
0
in the Sweep Gas Flow Rate
text box.
e.
Set the ESI spray voltage to 0:
In the ESI Source dialog box, enter
in the Spray Voltage text box.
f.
Click on
OK
.
7. Set up and start a flow of 50:50 methanol:water solution from the LC to
the API source, as follows:
a.
In the LTQ Tune Plus window, choose
Setup > Inlet Direct
Control
(or click on the AS/LC direct control button). The Inlet
Direct Control view appears.
b. Select the LC tab.
c.
Set the Flow Rate to a value that is typical for your experiments.
d. Set the solvent proportions to 50% methanol and water.
e.
Click on
(or
Pump On
or
Start Pump)
to start the LC pump.
8. Let the solution flow through the sample transfer line, sample tube, and
API probe for 15 min. After 15 min, turn off the flow of liquid from the
LC to the API source, as follows. Leave the API source (including the
APCI vaporizer, sheath gas, and auxiliary gas) on for an additional 5 min.
Click on
(or
Pump Off
or
Stop Pump)
to stop the LC pump.
