Daily Operation
Things to Do After Operating the LTQ
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3-6
_______________________ Finnigan LTQ Hardware Manual _______________________
3.2 Things to Do After Operating the
LTQ
After operating the LTQ you need to do the following:
•
Flush the sample transfer line, sample tube, and API probe
•
Place the system in standby condition
•
Flush the ion sweep cone and ion transfer capillary
•
Purge the oil in the rotary-vane pump
•
Empty the solvent waste bottle
Flushing the Sample Transfer Line, Sample
Tube, and API Probe
You should flush the sample transfer line, sample tube, and API probe at the
end of each working day (or more often if you suspect they are contaminated)
by flowing a 50:50 methanol:distilled water solution from the LC through the
API source.
To flush the sample transfer line, sample tube, and API probe, proceed as
follows:
1. Wait until data acquisition, if any, is complete.
2. Make sure that the lid to the API chamber in closed and secured.
3. Choose
Start > Programs > Xcalibur > LTQ Tune
to open the LTQ
Tune Plus window.
4. From the Tune Plus window, choose
Control > On
(or click on the
On/Standby button to toggle it to On) to turn on the voltages and gas
flows to the API source.
•
If you are operating in APCI or APPI mode, go to step 5.
•
If you are operating in ESI mode, go to step 6.
5. Set up the APCI source as follows:
a.
In the LTQ Tune Plus window, choose
Setup > APCI Source
to (or
click on the APCI source button) to display the APCI Source dialog
box.
b. Set the APCI vaporizer temperature to 500 °C:
In the APCI Source dialog box, enter
500
in the Vaporizer
Temperature text box.
c.
Set the sheath gas flow rate to 30:
In the APCI Source dialog box, enter
30
in the Sheath Gas Flow Rate
text box.
On Standby
