Thermo Scientific Thermo Scientific Vanquish VC-D40, Operating Manual

Page: 95 / 174

6 • Operation
Variable Wavelength Detectors (VC-D40, VF-D40)
Operating Manual Page 95
6.9.2 Long-Term Shutdown
Shutting Down the Device
To interrupt operation for a longer period, follow the instructions below.
TIP Shutting down the device affects the operation of the system. When
shutting down the device, also observe the shutting down instructions
for the other Vanquish system modules and take appropriate action
(refer to the
Operating Manuals for the modules).
1. Turn off both lamps.
2. Stop the pump flow.
3. Remove the column.
4. Connect the free ends of the column compartment capillaries using
a union connector (for example, the Viper union from the system
ship kit) and restart the pump flow.
5. Flush the flow cell with an appropriate solvent (minimum HPLC-
grade). Observe the following:
TIP With a Vanquish Core system that has been modified for using
normal-phase compatible solvents and additives, see the information
about the flushing liquid in the
Considerations with Normal-Phase
Compatible Solvents and Additives section in Operating Manual for the
pump.
Situation after Shutdown If no additive is used If an additive is used
Device and flow cell
remain in the laboratory
after shutdown Flush the system, for
example with methanol.
100% acetonitrile should
not be used. Flush the system with
several volumes of
methanol and water
(50:50) (for example,
1.0 mL/min for
10 minutes with the
standard system) to
prevent salt buildup in the
fluidics. If the solvents in
the flow cell are not
miscible with water, use
an appropriate
intermediate solvent.