Thermo Scientific Thermo Scientific Vanquish VC-D40, Operating Manual

Page: 145 / 174

8 • Troubleshooting
Variable Wavelength Detectors (VC-D40, VF-D40)
Operating Manual Page 145
8.3 Checking the Flow Cell
When
Related messages that require a flow cell check appear in the Audit Trail.
Follow these steps
1. Check that the flow cell is installed properly.
2. Remove and re-install the flow cell.
3. Flush the flow cell with pure HPLC-grade water with a flow rate of
>1 mL/min for several minutes (Nano flow cells: Flush with pure
HPLC-grade water with the maximum possible flow rate for the
respective range for several minutes).
4. Install a different flow cell and repeat the action during which the
message appeared with the flow cell that was previously installed.
Result Steps
If the message disappears with a
different flow cell The problems are caused by the flow cell
that was previously installed. If you
suspect a contamination or an elevated
backpressure, perform a flushing
procedure with the flow cell that was
previously installed (see Preparing the
Flow Cell for Storage).
If the message still appears The problem is caused by the flow path
or components in the flow path before
the detector or by the detector.
Install a diagnostic cell and repeat the
action during which the message
appeared with the flow cell that was
previously installed.
• If the message disappears with the
diagnostic cell
: The problems are
caused by the flow path or components
in the flow path before the detector.
• If the message still appears : The
problems are caused by the detector.
See also
2 Messages ( } page 140)