Thermo Scientific Thermo Scientific Vanquish VC-D40, Operating Manual

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9 • Specifications
Page 152 Variable Wavelength Detectors (VC-D40, VF-D40)
Operating Manual
For best performance, minimize the frequency and the amplitude of
changes in the ambient temperature to 1 °C/hour.
Time constant and response time
According to ASTM E1657-98, the time constant is converted to the
response time by multiplying by the factor 2.2 (i.e., response time =
2.2 x time constant).
9.1.2 Flow Cells
The flow cell specifications are as follows:
Type Path
length Illuminate
d volume Pressure
limit Materials in the
flow path Biocompatibility
Standard flow cell, SST 10 mm 11 µL 12 MPa Stainless steel,
fused silica, PEEK,
fluoropolymers,
titanium No
Standard Bio flow cell,
PEEK 10 mm 11 µL 5 MPa PEEK, Fused silica Yes
Semi-micro flow cell, SST 7 mm 2.5 µL 12 MPa Stainless steel,
fused silica, PEEK,
fluoropolymers,
titanium No
Semi-micro Bio flow cell,
PEEK 7 mm 2.5 µL 5 MPa PEEK, fused silica Yes
Semi preparative flow cell 0.4 mm 0.7 μL 10 MPa PEEK, fused silica Yes
Nano flow cell 10 mm* 3 nL 20 MPa Fused silica Yes
Capillary flow cell 10 mm* 45 nL 20 MPa Fused silica Yes
Mic flow cell 10 mm* 180 nL 20 MPa Fused silica Yes
* There is no exact definition of the light path in capillary flow cells as is for conventional
flow cells. Therefore, you should not use this value for calculating the absorption
coefficient or the detector sensitivity according to the Lambert-Beer law.