Thermo Scientific Thermo Scientific Vanquish VC-D40, Operating Manual

Page: 32 / 174

3 • Device Overview
Page 32 Variable Wavelength Detectors (VC-D40, VF-D40)
Operating Manual
3.1 Detector Features
The detector comprises the following main features:
• The detector is a dual-beam, variable wavelength photometer with
one measurement and one internal reference beam.
• Two different light sources provide a wavelength detection range
from 190 nm to 750 nm (VC-D40) and from 190 nm to 900 nm (VF-
D40):
¨
A deuterium lamp (referred to as UV lamp) for ultraviolet and
visible detection and
¨
A tungsten lamp (referred to as VIS lamp) for visible and near-
infrared detection (optional for VC-D40)
• Internal validation of the wavelength accuracy with holmium oxide
filter
• Multi-Wavelength Operation for additional operation functions,
measuring simultaneously at up to two (VC-D40) or four (VF-D40)
different wavelengths
• Data collection rates of up to 125 Hz (VC-D40) and up to 250 Hz (VF-
D40) under Chromeleon 7.2 software or later, in single-channel
mode
• Spectra measurements
• Two optical filters for eliminating higher order light
To ensure optimal measurement results over the entire wavelength
range, the optical filters automatically move into the light path (also
in multi-wavelength operation).
• Automatic wavelength calibration after power-up and when the
lamp configuration was changed.
• A shutter (motorized filter paddle) to move into the light path before
the flow cell
The shutter can move into the following positions:
¨
Open position for data acquisition
¨
Closed (dark) position for protection of the flow cell and for dark
signal measurements
¨
Holmium oxide filter position for validation of wavelength
accuracy
• Availability of flow cells for different fields of applications (for
details, see
Flow Cells (
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page 152) )