45
Thermo Fisher Scientific
Operation Manual HM550
• e specimen clamping moves forwards until the knife edge touches
the specimen.
• Immediately afterwards, this forward travel of the specimen clam-
ping is stopped and moved backwards by a safety distance of 200
µm. AUT.APPROACH is still shown on the display as the process
of the automatic approach has not yet been finished.
• When passing the next upper reversal point, the specimen clamping
automatically moves forwards again by 200 µm.
• e red LED in button (fig. 25.2) goes off as well as the term AUT.
APPROACH disappears.
• e specimen is now in position to start sectioning.
• Press button (fig. 25.2) or
• pass upper or lower reversal point via hand wheel.
When an automatic approach has been carried out, another approach
can be started immediately, if needed.
is might become necessary, when the specimen has been oriented
again.
•
Error code ‚AMPLIFIER ERROR‘
is shown on the status line on the display, in case the activating sig-
nal for the automatic approach has already been recognized before
the automatic approach movement has been started.
• Possible cause: the specimen has already been in contact with the
knife edge, e.g. by protruding fibers or the Cold D cycle was inter-
rupted.
• Press button (fig. 25.2), remove the cause for the error and press
button (fig. 25.2) again, to start the automatic approach once more.
• However, if there is no obvious explanation for this error code, e.g.
frost built-up, please call a service technician.
•
Error code ‚OUT OF TEMPERATURE‘
appears in the status line on the display, if the actual temperature of
the knife or the specimen is outside the temperature range of -5°C
and -35°C, which is valid for the automatic approach function only.
• Press button (fig. 25.2). e error message goes off.
Interruption of the automatic
approach
Error codes during an automatic
approach process
Operating instructions
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