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Thermo Fisher Scientific
Operation Manual HM550
e vacuum for stretching sections is turned off immediately after
reaching the end of the specimen via an in-line valve, however, depend-
ing on a correct setting of the cutting/suction window. Turn the vacuum
anti-roll hood backwards to transfer the cut onto a slide.
e Vacutome is also highly suitable for the disposal of debris.
For this, the vacuum anti-roll hood is used as described above. When
the anti-roll hood is put on, an air stream is generated in the suction
window.
Continue cutting and the penultimate section is pushed forwards and
reaches the area of the suction slot. From here the section gets through
the blade carrier and the tubing into the filter unit. Section waste is
collected in the coarse filter which can easily be replaced. It must be
disposed of according to the respective lab regulations.
e air of the suction flow now passes the micro filter and is filtered
there. Afterwards, the filtered air flows back into the rear area of the
cryo-chamber.
e specimen fast cooling and temperature control serves for a more
efficient working with the cryostat. For various specimens, different cut-
ting temperatures can be achieved very quickly.
ere is the possibility to rapidly freeze-on the specimen at -50°C on a
chuck with the freezing medium.
However, the freezing-on can also be carried out on the fast freezing sta-
tions or with the Peltier cooling element.
To bring the specimen to the desired cutting temperature, switch over
to temperature control. e set value of the cutting temperature can be
selected on the operating controls.
e specimen fast cooling and temperature control works with a refrig-
eration unit and an electronic control unit. e coolant (brine) flows
through the cooling head of the system and cools it.
While sectioning, the pre-selected cutting temperature is kept constant
by means of a corresponding control unit.
e specimen can be detached directly on the specimen head:
• Set specimen temperature from 0°C to 5°C (inside the chamber).
e following two processes are also possible:
• Active detach function, in case the instrument is equipped with this
accessory (inside the chamber).
• Cooling block with 6 stations for one chuck each (outside the
chamber.
Option O –
Specimen fast and
temperature control
Theory of operation
Option O – Specimen fast and temperature control
