Thermo Scientific LCQ Fleet, Getting Started Manual

Page: 54 / 74

6
Connecting the Inlet Plumbing
Sample Introduction
40 LTQ Series Getting Connected Guide Thermo Scientific
• For automated injections using an autosampler, connect the outlet from a liquid
chromatography system that contains an autosampler to port 2 of the divert/inject valve.
Then set up an autosampler to make automated injections into the solvent flow produced
by the LC pump.
Table 8 summarizes the sample introduction and analytical techniques for ESI/MS and
APCI/MS.
Table 8. Sample introduction and analytical techniques for ESI/MS and APCI/MS
Sample introduction into
the MS detector ESI analytical
technique APCI analytical
technique Procedure for connecting the
plumbing
Direct infusion Analysis of a pure
analyte
Automatic calibration
and tuning
“Setting Up the Inlet for Direct
Infusion”
on page 43
High-flow infusion
(syringe pump injection into
LC solvent flow) Analysis of a pure
analyte Analysis of a pure
analyte “Setting Up the Inlet for
High-Flow Infusion”
on
page 45
Loop injection into LC solvent
flow Analysis of a pure
analyte
Automatic
optimization of tuning
using an analyte
Analysis of a pure
analyte
Automatic
optimization of tuning
using an analyte
“Setting Up the Inlet for Loop
Injections (Flow Injection
Analyses)”
on page 51
LC system with autosampler
(without chromatographic
separation) Analysis of one or more
pure analytes Analysis of one or more
pure analytes “Setting Up the Inlet for an
LC/MS System with an
Autosampler”
on page 53
LC system with autosampler
(with LC column for
chromatographic separations) Analysis of a mixture Analysis of a mixture “Setting Up the Inlet for an
LC/MS System with an
Autosampler”
on page 53