Thermo Scientific LCQ Fleet Getting Started Manual Download Page 9

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Thermo Scientific

LTQ Series Getting Connected Guide

Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xi

About This Guide. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xi
Safety and Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xii
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xiii

Chapter 1

Line Power, Vacuum System, Gases, and Ethernet Communication. . . . . . . . . . .1

Connecting the MS Detector to Line Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Connecting the Forepump to the LCQ Fleet or LXQ MS Detector . . . . . . . . . . 2

Connecting the Vacuum Hose. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Connecting the Forepump to the Laboratory Exhaust System . . . . . . . . . . . . . 4
Connecting the Forepump to Line Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5

Connecting the Forepumps to the LTQ XL MS Detector . . . . . . . . . . . . . . . . . . 6
Connecting the Gases to the MS Detector. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7

Connecting the Nitrogen Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Connecting the Helium Source . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8

Connecting the MS Detector to the Data System Computer. . . . . . . . . . . . . . . . 9

Chapter 2

Connecting Probes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11

Connecting the ESI Probe to the MS Detector . . . . . . . . . . . . . . . . . . . . . . . . . 11
Connecting the APCI Probe to the MS Detector. . . . . . . . . . . . . . . . . . . . . . . . 12

Chapter 3

Connecting the MALDI LTQ XL System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13

Connecting the MALDI LTQ XL System Forepumps to Line Power . . . . . . . . 14
Connecting the MALDI LTQ XL Vacuum System . . . . . . . . . . . . . . . . . . . . . . 15
Connecting the MALDI Control Module to the MALDI LTQ XL System. . . . 17
Connecting the MALDI CCD Camera to the Data System Computer . . . . . . . 20

Chapter 4

Connecting External Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23

External Devices Controlled by the Xcalibur Data System. . . . . . . . . . . . . . . . . 24

Making Contact Closure with Devices Controlled by Xcalibur . . . . . . . . . . . 24
Selecting the Appropriate Start Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . 25

External Devices Not Controlled by the Xcalibur Data System . . . . . . . . . . . . . 27

Making Contact Closure with Devices Not Controlled by Xcalibur . . . . . . . 27
Starting a Sequence Run from Xcalibur . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28

Contents

Summary of Contents for LCQ Fleet

Page 1: ...LTQ Series Getting Connected Guide 97055 97071 Revision A January 2008 ...

Page 2: ... provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproduction of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document ...

Page 3: ...ntific or one of its authorized representatives This document contains the testing and evaluation results for these Thermo Scientific products LCQ Fleet March 2007 LXQ February 2005 LTQ XL September 2006 LTQ XL with the ETD module January 2007 MALDI LTQ XL August 2007 LCQ Fleet March 2007 EMC Directive 89 336 EEC as amended by 92 31 EEC and 93 68 EEC EMC compliance has been evaluated by TUV Rheinl...

Page 4: ...fic sole responsibility This device complies with Low Voltage Directive 73 23 EEC and harmonized standard EN 61010 1 2001 EN 55011 1998 EN 61000 4 3 2002 A1 2002 EN 61000 3 2 1995 A1 1998 A2 1998 A14 2000 EN 61000 4 4 1995 A1 2001 A2 2001 EN 61000 3 3 1995 EN 61000 4 5 1995 A1 2001 EN 61326 1 1997 EN 61000 4 6 1996 A1 2001 EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 11 1994 A1 2001 FCC Class A CF...

Page 5: ... 95 EC and harmonized standard EN 61010 1 2001 Safety of Laser Products Compliance with safety of laser products is declared under Thermo Fisher Scientific sole responsibility This device complies with the harmonized standard IEC EN 60825 1 A2 2001 EN 61000 3 2 1995 A1 1998 A2 1998 A14 2000 EN 61000 4 4 1995 A1 2000 A2 2001 EN 61000 3 3 1995 A1 2001 EN 61000 4 5 1995 A1 2001 EN 61326 1 1997 A1 199...

Page 6: ...d by the instrument Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturing location see the label on the instrument THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES OPERATION IS SUBJECT TO THE F...

Page 7: ... Directives and the recyclers in your country WEEE Konformität Dieses Produkt muss die EU Waste Electrical Electronic Equipment WEEE Richtlinie 2002 96 EC erfüllen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder ents...

Page 8: ...ieurs compagnies de recyclage dans chaque état membre de l union européenne et ce produit devrait être collecté ou recyclé par celles ci Davantage d informations sur la conformité de Thermo Fisher Scientific à ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la détection des substances sujettes à la directive RoHS sont di...

Page 9: ... the MS Detector to the Data System Computer 9 Chapter 2 Connecting Probes 11 Connecting the ESI Probe to the MS Detector 11 Connecting the APCI Probe to the MS Detector 12 Chapter 3 Connecting the MALDI LTQ XL System 13 Connecting the MALDI LTQ XL System Forepumps to Line Power 14 Connecting the MALDI LTQ XL Vacuum System 15 Connecting the MALDI Control Module to the MALDI LTQ XL System 17 Connec...

Page 10: ...e Syringe 44 Connecting an Infusion Line to the Grounding Union 44 Setting Up the Inlet for High Flow Infusion 45 Connecting the Syringe to the Union Tee 46 Connecting the Union Tee to the Divert Inject Valve 47 Connecting the LC Pump to the Divert Inject Valve 48 Connecting the Divert Inject Valve to a Waste Container 48 Connecting the Union Tee to the Ion Source 49 Setting Up the Inlet for Loop ...

Page 11: ...ociation by transferring electrons to positively charged peptides leading to a rich ladder of sequence ions derived from cleavage at the amide groups along the peptide backbone Amino acid side chains and important modifications such as phosphorylation are left intact The LTQ XL MALDI system an LTQ XL mass spectrometer pre configured for compatibility with the MALDI source The optional API kit allo...

Page 12: ...Q Fleet LCQ Fleet Getting Started Guide LCQ Fleet Hardware Guide LTQ XL LTQ XL Getting Started Guide LTQ XL Hardware Guide LTQ XL ETD MS ETD System Getting Started Guide MS ETD System Getting Connected Guide MS ETD System Hardware Guide LXQ LXQ Getting Started Guide LXQ Hardware Guide MALDI Source MALDI Source Hardware Manual MALDI Source Getting Started Guide API Source Ion Max and Ion Max S API ...

Page 13: ...Y To contact Customer Service for ordering information Y To suggest changes to documentation or to Help Fill out a reader survey online at www thermo com lcms techpubs Send an e mail message to the Technical Publications Editor at techpubs finnigan lcms thermofisher com Phone 800 685 9535 Fax 561 688 8736 E mail TechSupport C MS thermofisher com Knowledge base www thermokb com Phone 800 532 4752 F...

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Page 15: ...ptacle of the power panel 4 Connect the male plug of the power cord to the 230 V ac power source in your laboratory Contents Connecting the MS Detector to Line Power Connecting the Forepump to the LCQ Fleet or LXQ MS Detector Connecting the Forepumps to the LTQ XL MS Detector Connecting the Gases to the MS Detector Connecting the MS Detector to the Data System Computer CAUTION If your local area i...

Page 16: ...e two ends of the vacuum hose assembly are connected at the factory Table 1 Vacuum hose accessory kit P N 97055 60135 Description Part Number Vacuum hose 1 5 in ID 8 ft length 00301 24163 Parts required to connect the vacuum port of the forepump Centering ring with O ring NW25 26 mm ID aluminum and Viton 00108 02011 Swing clamp KF 20 25 00102 10020 Hose clamp high torque 1 25 in to 2 125 in stainl...

Page 17: ... that has the adapter for the MS detector to the vacuum port on the back of the MS detector 2 To connect the other end of the vacuum hose to the forepump a Place the 26 mm 1 0 in ID centering ring P N 00108 02011 on the flange of the inlet port of the forepump b Using the KF20 25 vacuum clamp P N 00102 10020 secure the vacuum hose assembly to the forepump Figure 2 on page 4 shows the connection be...

Page 18: ...ese solvents The frequency of the purging depends on the throughput of your system Y To connect the exhaust port of the forepump to the laboratory exhaust system 1 Use hose clamps P N 00108 09001 to secure the 2 5 cm 1 in ID blue exhaust hose to the forepump exhaust port 2 Vent the free end of the blue exhaust hose to an external exhaust system in your laboratory The exhaust hose should travel at ...

Page 19: ...ect the forepump to line power the forepump outlet of the MS detector 1 On the power panel switch the Main Power circuit breaker to the Off position 2 Connect the power cord plug of the forepump to the Mech Pumps power receptacle on the MS detector 3 Turn on the power switch of the forepump Figure 3 View of the line power connection for the forepump mechanical pump Reset Ethernet 100 Base T Main P...

Page 20: ...ing P N 00108 02011 on the flange of each forepump vacuum inlet 6 Connect the vacuum hose with the attached fitting adapter to the pump vacuum inlet Secure the hose to the pump using the KF20 25 vacuum hardware clamp P N 00102 10020 7 Use hose clamps P N 00108 09001 to connect the 2 5 cm 1 in ID blue exhaust hoses from the forepump exhaust ports to the pump exhaust system in your laboratory Becaus...

Page 21: ...ium Source Connecting the Nitrogen Source The LTQ Series MS detector requires high purity 99 nitrogen for the API sheath gas auxiliary gas and sweep gas Because nitrogen gas usage can be quite high Thermo Fisher Scientific recommends one of three nitrogen sources a large sealed thermally insulated cylinder containing liquid nitrogen from which the nitrogen gas is boiled off the largest nitrogen cy...

Page 22: ...5 70 kPa 40 10 psi Because particulate filters can be a source of contamination Thermo Fisher Scientific does not recommend their use You can dispense helium from a tank containing 245 ft3 of gas using a Matheson 3120 Series1 regulator or equivalent tank and regulator Whether you choose copper or stainless steel gas lines for the helium use gas lines that are free of oil and preferably flame dried...

Page 23: ...ith the data system computer through an Ethernet cable Y To connect the MS detector to the data system computer 1 Connect a category five network Ethernet cable P N 00302 01838 to the ETHERNET 100 BASE T connector located on the MS detector power panel 2 Connect the opposite end of the Ethernet cable to the 10 100 Base T Ethernet switch P N 00825 01015 provided with the MS detector 3 Connect a sec...

Page 24: ......

Page 25: ...e container and vent the waste container to a fume exhaust system Contents Connecting the ESI Probe to the MS Detector Connecting the APCI Probe to the MS Detector CAUTION Prevent solvent waste from backing up into the API source and mass spectrometer Always ensure that the PVC drain tubing is above the level of liquid in the waste container Do not vent the PVC drain tube connected to the waste co...

Page 26: ...nt the waste container to a fume exhaust system CAUTION Prevent solvent waste from backing up into the API source and mass spectrometer Always ensure that the PVC drain tubing is above the level of liquid in the waste container Do not vent the PVC drain tube connected to the waste container to the same fume exhaust system to which you have connected the forepump Connecting the API source drain tub...

Page 27: ...ter describes the external cable connections for a MALDI LTQ XL system Contents Connecting the MALDI LTQ XL System Forepumps to Line Power Connecting the MALDI LTQ XL Vacuum System Connecting the MALDI Control Module to the MALDI LTQ XL System Connecting the MALDI CCD Camera to the Data System Computer ...

Page 28: ... you connect the primary forepump to the power entry panel of the LTQ XL MS detector and the secondary forepump to the MALDI control module Figure 6 Line power connections for the forepumps Vacuum Valve Motors Laser Comm Sensors Laser Power Aux DISCONNECT WARNING MODEL MALDI Laser Control Module SERIAL No CAUTION Finnigan MAT PASS Ground Continuity and HPOT Tests Date By Power Power In From LTQ Ac...

Page 29: ...system Figure 7 shows the components of the MALDI solenoid vacuum valve and vacuum hose assembly Figure 7 MALDI solenoid vacuum valve assembly CAUTION Only Thermo Fisher Scientific field service engineers or personnel who have received equivalent training from Thermo Fisher Scientific on installing the MALDI source can connect the forepumps to the MALDI LTQ XL system Table 2 Kits required to conne...

Page 30: ...s Vacuum Valve EDWARDS 30 Oil Mist Filter EMF 20 EDWARDS EDWARDS 30 Oil Mist Filter EMF 20 EDWARDS Power connection between the secondary forepump and the MALDI control module Power connection between the primary forepump and the MS detector power entry panel Vacuum connection between the LTQ XL MS detector and the primary forepump Vacuum connection between the MALDI sample module and the secondar...

Page 31: ...LDI control module to the MALDI sample module The MALDI control module gets its line power from the LTQ XL MS detector Table 3 Cables connecting the MALDI control module and the LTQ XL MALDI system not including the power cable Part Number Description 97155 63002 Motors cable 97155 63082 Sensor cable 97155 63026 Laser communications cable 97155 63101 Laser power cable 97155 63102 MALDI LTQ Comm ca...

Page 32: ...the right side of the LTQ XL MS detector Figure 11 shows the cable connection between the back side of the MALDI optics module and the MALDI control module Figure 9 Connections between the left side of the MALDI LTQ XL system and the MALDI control module Sensors Motors CCD Camera Laser Power Laser Comm Vacuum Valve Motors Laser Comm Sensors Laser Power Aux DISCONNECT WARNING MODEL MALDI Laser Cont...

Page 33: ...onnection between the MALDI control module and the LTQ XL power entry panel DISCONNECT WARNING MODEL MALDI Laser Control Module SERIAL No CAUTION Finnigan MAT PASS Ground Continuity and HPOT Tests Date By Power Power In From LTQ Accessory Outlet ONLY OFF ON Vacuum Pump MALDI LTQ XL system right side panel MALDI control module rear panel Power cable P N 00302 05 00002 ...

Page 34: ...3 show a video signal cable P N 97155 63110 connects the video output from the MALDI CCD camera to the data system computer Figure 12 Video signal cable l Vacuum Valve Motors Laser Comm Sensors LaserPower Aux DISCONNECT WARNING MODEL MALDI Laser Control Module SERIAL No CAUTION Finnigan MAT PASS Ground Continuity and HPOT Tests Date By Power Power In From LTQ Accessory Outlet ONLY OFF ON Vacuum Pu...

Page 35: ...1 Figure 13 Video signal cable connection Sensors Motors CCD Camera Laser Power Laser Comm Video signal cable Osprey cable MALDI LTQ XL system left side panel Data system computer rear panel Connect one end of the video signal cable to the yellow monitor connector of the Osprey cable Connect the Osprey cable connector to the the computer s video port ...

Page 36: ......

Page 37: ...Kit Description of Kit OPTON 21705 Xcalibur contact closure kit for devices not controlled by Xcalibur 2 wire trigger cable 8 position screw connector OPTON 21709 Xcalibur Additional 4 Port Serial Kit 4 port serial PCB PCI and software Quad DB9 male adapter OPTON 21710 Xcalibur Waters Interface Kit Waters serial I F cable 2 wire trigger cable contact closure OPTON 21721 Xcalibur SS420x Interface K...

Page 38: ...y Xcalibur Selecting the Appropriate Start Instrument Making Contact Closure with Devices Controlled by Xcalibur Thermo Fisher Scientific provides instructions for connecting supported liquid chromatography systems to your Thermo Scientific mass spectrometer You can access the appropriate instruction guide from the data system computer Y To connect the contact closure cable 1 Connect the appropria...

Page 39: ... click the Sequence Setup button The Sequence Setup window appears 2 Open the sequence that you want to run a Choose File Open The Open dialog box appears b Browse to the appropriate folder and then select a sequence file c Click Open to open the sequence and exit the Open dialog box Sequence files are identified by their sld file extension 3 Choose Actions Run Sequence or Actions Run This Sample ...

Page 40: ...ppropriate device is listed as the start instrument in the Acquisition Options box 5 If the appropriate device is not listed as the start instrument change the starting device a Click Change Instruments The Change Instruments In Use dialog box appears with for example the Surveyor Autosampler selected as the Start instrument See Figure 15 Figure 15 Change Instruments In Use dialog box with the Sur...

Page 41: ...LTQ Series MS detector follow these procedures Making Contact Closure with Devices Not Controlled by Xcalibur Starting a Sequence Run from Xcalibur Making Contact Closure with Devices Not Controlled by Xcalibur Y To connect the contact closure cable 1 Connect the 2 wire trigger cable in kit P N OPTON 21705 to the Start In pins on the power entry panel of the LTQ Series MS detector 2 Connect the ca...

Page 42: ...s family of MS detectors consists of the LCQ Fleet LXQ LTQ and LTQ XL MS detectors Y To start the sequence run 1 On the Xcalibur Roadmap Home Page click the Sequence Setup button to open the Sequence Setup window 2 Open the sequence that you want to run a Choose File Open The Open dialog box appears b Browse to the appropriate folder and then select a sequence file c Click Open to open the sequenc...

Page 43: ...s MS detector is the only configured instrument by default it is listed as the start instrument for the sequence run Figure 17 shows Yes under Start Instrument for the LCQ Fleet MS detector Figure 17 Run Sequence dialog box with the MS detector specified as the start instrument 4 Click Change Instruments The Change Instruments In Use dialog box appears See Figure 18 ...

Page 44: ...rument method downloads to the LTQ Series and the Status page displays Waiting Contact Closure If the Roadmap Home Page does not display the Info view click the Information View button to display it and then click the Status tab to display the Status page 7 Start the external device Acquisition from the LTQ Series MS detector begins after the external device sends the Contact Closure signal that t...

Page 45: ...Connected Guide 31 Figure 19 SpectraSYSTEM LC system P4000 SpectraSYSTEM ENTER MENU ST ATUS RUN STOP PURGE Pump UV2000 SpectraSYSTEM ENTER MENU ST ATUS RUN STOP ZERO Detector SpectraSYSTEM ENTER MENU ST ATUS RUN STOP SAMPLES AS3000 Autosampler SpectraSYSTEM Degasser Front panel command center Degasser Pump Autosampler Detector ...

Page 46: ......

Page 47: ...mation on making the plumbing and back panel connections for a Thermo Scientific LC system refer to its getting connected manual Note The 7 connector LC MS interconnect cable is described in this chapter For instructions on using the 5 connector LC MS interconnect cable refer to the Surveyor Plus Getting Connected Guide Contents Contact Closure Cables Making Contact Closure with a Surveyor Plus LC...

Page 48: ...Surveyor MS Pump or Surveyor MS Pump Plus you must also have the MS pump adapter cable shown in Figure 22 Figure 20 LC MS interconnect cable with seven combicom connectors P N 60053 63034 Figure 21 LTQ LXQ interconnect adapter cable P N 60053 63037 Figure 22 MS pump adapter cable P N 60053 63034 for the Surveyor MS Pump or Surveyor MS Pump Plus DETECTOR DETECTOR DETECTOR PUMP PUMP A S M S RED BLAC...

Page 49: ...m modules the LTQ LXQ interconnect adapter cable and the MS pump adapter cable for systems with a Surveyor MS Pump or Surveyor MS Pump Plus The LTQ LXQ interconnect adapter cable P N 60053 63037 and its connections to the 7 connector LC MS interconnect cable and the LTQ Series MS detector The MS pump adapter cable P N 60053 63038 and its connections to the 7 connector LC MS interconnect cable and ...

Page 50: ...Pump or Surveyor MS Pump Plus Figure 23 Surveyor Plus LC system connected to an LTQ Series MS detector Start In DETECTOR DETECTOR DETECTOR PUMP PUMP A S PUMP MS PUMP MS Detector Autosampler Pump Detector Detector 7 connector LC MS interconnect cable Pump Detector Solvent platform Autosampler rear panel Surveyor LC Pump rear panel Detector rear panel LTQ Series MS detector right side panel LTQ LXQ ...

Page 51: ...system to the LTQ Series MS detector you must have the LTQ LXQ interconnect adapter cable P N 60053 63037 shown in Figure 21 in addition to the 7 connector LC MS interconnect cable P N 60053 63034 Y To connect an LTQ Series MS detector to an Accela LC system 1 Connect the 8 pin connector with hood labeled MS LC end of the LTQ LXQ interconnect adapter cable to the connector labeled M S of the 7 con...

Page 52: ...a LC connected to an LCQ Fleet MS detector Start In DETECTOR DETECTOR DETECTOR PUMP PUMP A S MS Detector Autosampler Pump Detector 7 connector LC MS Interconnect cable Pump Detector Solvent platform Autosampler rear panel Pump rear panel PDA detector rear panel LTQ Series MS detector right side panel LTQ LXQ interconnect adapter cable Detector Not drawn to scale ...

Page 53: ...e ion source To introduce sample into the ion source set the rate at which the syringe pump depresses the plunger of the syringe and the flow rate of the solvent stream produced by the LC pump For loop injection flow injection analyses connect the solvent flow from an LC pump to port 2 of the divert inject valve a sample loop to ports 1 and 4 of the valve and a loop filler to port 5 of the valve T...

Page 54: ...utomatic calibration and tuning Setting Up the Inlet for Direct Infusion on page 43 High flow infusion syringe pump injection into LC solvent flow Analysis of a pure analyte Analysis of a pure analyte Setting Up the Inlet for High Flow Infusion on page 45 Loop injection into LC solvent flow Analysis of a pure analyte Automatic optimization of tuning using an analyte Analysis of a pure analyte Auto...

Page 55: ...eedle Kit contains a blunt tip 32 gauge stainless steel needle ferrules PEEK adapter union and zero dead volume ZDV 1 4 28 union OPTON 20014 Metal Needle Kit contains a blunt tip 34 gauge stainless steel needle ferrules PEEK adapter union and ZDV 1 4 28 union OPTON 20015 Tubing fused silica 0 1 mm ID 0 4 mm OD infusion line 00106 10504 Tubing fused silica 0 1 mm ID 0 190 mm OD fused silica sample ...

Page 56: ...tting adapter union PEEK Upchurch Scientific natural used with blunt tip 32 or 34 gauge stainless steel needle included in Metal Needle Kit 00101 18206 Nut LC for 1 16 in stainless steel Rheodyne 2522 0066 Ferrule LC for 1 16 in OD high pressure tubing Rheodyne used to connect tubing and the sample loop to the divert inject valve 2522 3830 5 μL sample loop stainless steel Rheodyne 00110 22026 10 μ...

Page 57: ...e To introduce sample solution with the syringe pump you must connect an infusion line between the syringe pump and the grounding union that is held by the grounding bar of the Ion Max S API source See Figure 25 To connect the syringe to the grounding union follow these procedures 1 Setting Up the Syringe 2 Connecting an Infusion Line to the Grounding Union Figure 25 Connection between the ESI pro...

Page 58: ...re 26 Connecting the syringe and the LC union 4 Place the syringe into the syringe holder of the syringe pump 5 While squeezing the blue release buttons on the syringe pump handle push the handle forward until it just contacts the syringe plunger Connecting an Infusion Line to the Grounding Union Y To connect an infusion line between the LC union and the grounding union 1 Connect a section of red ...

Page 59: ...umbing connections for sample introduction from the syringe pump into solvent flow from an LC pump perform these procedures in any order Connecting the Syringe to the Union Tee Connecting the Union Tee to the Divert Inject Valve Connecting the LC Pump to the Divert Inject Valve Connecting the Divert Inject Valve to a Waste Container Connecting the Union Tee to the Ion Source Grounding union 10 32 ...

Page 60: ...he Syringe on page 44 2 Using a fingertight fitting and a ferrule connect a red PEEK infusion line to the free end of the LC union that is connected to the syringe 3 Using a fingertight fitting and a ferrule connect the other end of the red PEEK infusion line to the union Tee Figure 28 shows the fittings required to connect the LC union to the union Tee Figure 28 Connecting the LC union to the uni...

Page 61: ... length of red PEEK tubing to port 3 of the divert inject valve Or use a stainless steel nut and ferrule to connect the tubing to the divert inject valve 2 Using a fingertight fitting and a ferrule connect the other end of the tubing to the free end of the union Tee See Figure 29 and Figure 30 Figure 29 Six port divert inject valve connections 6 5 4 3 2 1 6 5 4 3 2 1 To LC TEE union To waste From ...

Page 62: ...f the divert inject valve 2 Using an appropriate fitting and ferrule connect the other end of the tubing to the outlet of the LC Connecting the Divert Inject Valve to a Waste Container Y To connect the divert inject valve to a waste container 1 Using a fingertight fitting and a ferrule connect a length of red PEEK tubing to port 1 of the divert inject valve Figure 29 shows the ports of the divert ...

Page 63: ...re 31 Plumbing diagram showing APCI MS sample introduction with high flow infusion For the ESI probe use a fingertight fitting and a ferrule to connect the other end of the tubing to the grounding union that is held by the grounding bar of the Ion Max S API source See Figure 32 and Figure 33 The grounding union slides into the grounding bar on the Ion Max S API source as shown in Figure 38 on page...

Page 64: ...e grounding union used for the ESI probe Figure 33 Plumbing diagram showing ESI MS sample introduction with high flow infusion Ferrules P N 00101 18196 Grounding union Fingertight fittings P N 00101 18081 PEEK tubing P N 00301 22912 Syringe 3 2 1 From TEE to grounding union From port 1 to waste container From syringe to TEE From LC pump outlet to port 2 From port 3 to TEE ...

Page 65: ...tting and ferrule connect one end of a length of red PEEK tubing to the outlet of the LC pump To produce a stable solvent flow the Surveyor MS Pump Plus requires a minimum backpressure of 3 bar 43 psi To connect the Surveyor MS Pump Plus use a length of 0 005 in ID PEEK tubing sufficient to exert a backpressure of 3 bar 43 psi or connect an in line backpressure regulator between the LC pump outlet...

Page 66: ...or the APCI probe use two fingertight fittings and two ferrules to connect a length of red PEEK tubing between port 3 of the divert inject valve and the sample inlet of the APCI probe See Figure 35 Figure 35 Plumbing diagram for loop injection into the solvent flow from an LC into an APCI probe 3 1 6 Load Inject Waste Detector Connection between port 2 of the divert inject valve and the LC pump Co...

Page 67: ...nt flow from an LC into an ESI probe 5 To connect the divert inject valve to a waste container Use a fingertight fitting and a ferrule to connect one end of a length of red PEEK tubing to port 6 of the divert inject valve Place the other end of the tubing to an appropriate waste container Setting Up the Inlet for an LC MS System with an Autosampler Y To connect the inlet plumbing for an LC MS syst...

Page 68: ...rt 3 of the divert inject valve and the grounding union To connect the other end of the grounding union to the ESI probe sample inlet follow the instructions in the Ion Max and Ion Max S API Source Hardware Manual For the APCI probe use two fingertight fittings and two ferrules to connect a length of red PEEK tubing between port 3 of the divert inject valve and the APCI probe sample inlet 4 To con...

Page 69: ...Grounding Union to the ESI Probe Sample Inlet For instruction on connecting the PEEK safety sleeve and fused silica sample tube from the grounding union to the ESI probe sample inlet refer to the Ion Max and Ion Max S API Source Hardware Manual Figure 38 Connecting the grounding union to the sample inlet of the ESI probe Sample inlet Grounding bar Grounding union ...

Page 70: ......

Page 71: ...ports for the data system computer See Figure 39 Table 10 lists the kit and its contents used with the 4 port serial PCB Figure 39 4 port serial PCB and quad DB9 male cable Table 10 Kit used with the 4 Port Serial PCB Part number Description of kit OPTON 21709 Xcalibur Additional 4 Port Serial Kit 4 port serial PCB PCI and software Quad DB male adapter 4 port serial PCB Quad DB9 male cable ...

Page 72: ...e computer 6 Plug the 4 port serial PCB into the slot of the computer by firmly pushing the edge of the card into the connector until the card is seated 7 To secure the 4 port serial PCB in place use the screw from the slot cover plate 8 Replace the computer cover 9 To connect the quad DB9 male cable a Connect the SCSI port connector of the quad DB9 male cable to the connector located on the 4 por...

Page 73: ...netic compatibility iii Electronics switch 1 exhaust system connecting forepump to 4 external devices controlled by Xcalibur contact closure 24 not controlled by Xcalibur contact closure 27 F fittings 41 flow injection analysis setting up the plumbing 51 forepump connecting exhaust system 4 line power connecting 5 5 LTQ XL MS connecting to 6 MS detector connecting 6 MS detector vacuum port connect...

Page 74: ...mple loops 42 samples introducing 39 Serial PCB 4 Port 57 SS420x Xcalibur SS420x Interface Kit 23 start instrument selecting 25 setting 29 Surveyor Plus LC devices contact closure 35 syringe connecting to LC union 44 connecting to union Tee 46 syringe pump installing syringe into 44 plumbing 45 T trigger 2 wire contact closure connection for inlet devices not supported by Xcalibur 27 tubing 41 U u...

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