Chapter 2
Measuring Principle
2.3 Measuring Function description
·
The
chemically emitted light
is measured by a selected high-
sensitive, low-noise
photo multiplier [PMT]
. The linear
measuring range of the photo multiplier is 300 - 650 nm. The light
peak of the chemiluminescence is emitted at a wavelength of 420
nm.
·
The PMT is operating as an ultra-fast photo counter. The pulses
are amplified by a rapid electronic amplifier. A circuit, which
suppresses the PMT signal-noise is also implemented in the
PMT box.
·
Not the number of counts, but the
Relative Light Units [RLU]
are used as units of the measurement for the raw data, which is
then multiplied by the RLU factor, that allows the compensation of
the inevitable, individual fluctuations of the cathode sensitivity of
the PMT.
Page 2-4
Operating instructions