Chapter 2
Measuring Principle
2.2 Test procedure 2-step assay
A 2-step assay refers to an assay that has:
·
2 incubation sequences
(the time of incubation may
range depending on the assay).
·
2 wash sequences
(the amount of washing for each
sequence is assay dependent).
Most assays that are 2-step have an average incubation time of 10
minutes each. Fig. 2.1.2-1 is only an example. Pipetting
sequences are also assay dependent.
Fig. 2.2-1: Examples of test procedure “2-step assay”
Operating instructions
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