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Performing and Evaluating Measurements
Measuring Samples and Displaying the Measuring Results
6.2 Measuring Samples and Displaying the Measuring
Results
+
Rotate the recess in the sample platform until it points forwards and remove the CASY cup from
below the measuring capillary.
+
Place the sample to be measured below the measuring capillary and ensure that the external
platinum electrode is immersed in the sample.
+
Rotate the sample platform slightly to the other side again.
+
Place the protection shield around the sample platform including the capillary and the external
electrode by pushing it completely to the wall of the sample chamber.
+
Press
START
to perform a measurement using the current parameters. The CASY System auto-
matically works through all the necessary steps. After a short term, the size distribution for your
sample appears in the display.
Instructions for setting up the major applications can be found in the
CASY TT Customer Training
Guide
.The example above shows the size distribution
for an
eukaryotic cell line
.
+
To the left of the left-hand normalization cursor appearing as a dotted line (< 7.6 µm), you will
see the
Debris
Peak.
+
Between the left-hand normalization cursor and the left-hand evaluation cursor shown by a solid
line (7.6 to 12.8 µm), you will see the peak for the
dead cells
. In the case of dead cells, the cell
membrane no longer acts as an electrical barrier. Therefore dead cells are shown by the size of
their nucleus.
+
To the right of the left-hand evaluation cursor, the peak for the
viable cells
begins followed by
cell aggregates
.
The size distribution for your samples may appear differently, depending on your applications,
your cells and the used measuring capillaries.
