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OLS CASY TT, Operator'S Manual

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OLS CASY TT Operator'S Manual Download Page 17

17

Device configuration

Calibrated Vertical Tube

Pressure System  
(concealed)

Light-Emitting Diodes

Measuring Unit

External electrode

Protection Shield

Cable (connecting 
Protection Shield and 
the Measuring Unit)

Storage Container

Sample platform

Precision Measuring Capillary

Waste Container

Base

Power Supply

Data Socket (DB9)

Parallel port (DB25)

Serial port,  
RS232 (DB9)

Power Switch

Power input
socket

Voltage selector

Frontal view:

Rear view:

Figure 5: Schematic of CASY TT, frontal and rear view

Summary of Contents for CASY TT

Page 1: ...CASY TT CASY TT CELL COUNTER ANALYZER Operators Guide For life science research only Not for use in diagnostic procedures...

Page 2: ......

Page 3: ...s 12 1 2 3 Measuring Range Dynamics 13 1 3 Viability Determination using Electrical Current Exclusion 14 1 4 Aggregation Correction 15 1 5 CASY TT 15 2 Device configuration 16 2 1 Measuring Unit 18 2...

Page 4: ...Control Panel Keys 48 6 7 Outputting the Results 48 7 Troubleshooting 50 7 1 Error Messages 50 7 2 Background too High 51 7 3 Message Concentration too high 52 7 4 Message Measuring Timeout Capillary...

Page 5: ...Telefon 0421 27 61 69 0 e mail info ols bio de Online Support CASY online support is available on www cellcounting de direct online contact manuals literature setup files video manuals tips tricks tr...

Page 6: ...rks of OMNI Life Science GmbH Co KG Other brands or product names are trademarks of their respective holders VI Intended Use The CASY TT is intended for highly standardized cell counting and accurate...

Page 7: ...ndicate that noncompliance with instructions or procedures could lead to physical injury or even death or could cause damage to the instrument BIOHAZARD This symbol indicates that certain precautions...

Page 8: ...ed by authorized service technicians only Always ensure that there is sufficient CASY ton in the CASY ton storage container Clean the containers before reusing them Always observe the safety regulatio...

Page 9: ...ecomes cracked frayed broken or otherwise damaged replace it immediately with the equivalent part from OLS IX Disposal of the Instrument All electrical and electronic products should be disposed of se...

Page 10: ...ased on the cell volume the corresponding cell diameter is calculated Aggregation correction Determination of the mean degree of cell aggregation The aggregation factor is used to calculate the total...

Page 11: ...lyte developed specifically for cell counting For the measurement the cells are aspirated through a precision measuring pore of de fined geometry at a constant flow speed Signal Generation The precisi...

Page 12: ...ates the integral of the measuring signal from the individ ual readings The calculated signal areas are evaluated by a standardized method and accumulated in a multi channel analyzer From the original...

Page 13: ...ated by the CASY Cell Counter and Analyzer Based on the precisely determined cell volumes the CASY Cell Counter and Analyzer calculates the corresponding cell diameters which are displayed together wi...

Page 14: ...ire cell the populations debris dead cells and viable cells can be clearly distinguished in the high resolu tion size distribution For calculating the viability in the analyzed suspension two pairs of...

Page 15: ...be used as a further quality control parameter for the characterization of cell cultures For more information on using aggregation correction and determining the aggregation factor please visit www ce...

Page 16: ...on shield delivered with cable in the accessory box Control panel with graphic display and 13 control keys see Figure 6 Measuring unit pressure system and mains power supply are housed in three separa...

Page 17: ...hield Cable connecting Protection Shield and the Measuring Unit Storage Container Sample platform Precision Measuring Capillary Waste Container Base Power Supply Data Socket DB9 Parallel port DB25 Ser...

Page 18: ...sually is situated above the sam pling chamber The inspection window accommodates three light emitting diodes which indicate the filling level of the vertical tube The green light emitting diode indic...

Page 19: ...rew in the cap nut manually and do not use a tool Ensure that the tube extending inside the measuring capillary is not buckled or pinched during assembly Carefully rotate the exter nal electrode witho...

Page 20: ...de to the right see Figure 5 Special filters ensure that the mains power supply is afforded maximum interference protection 2 4 Control Panel The control panel see Figure 6 consists of a graphic displ...

Page 21: ...ases number change selection Increases number change selection PRINT Output to printer and or se rial interface CASY excell CLEAN Starts one or several cleaning cycles START Starts a measurement L CUR...

Page 22: ...tection an Uninterruptible Power Supplies UPS is recommended depending upon the quality of electrical grounding of the local electrical power supply Connect the analyzer to grounded power outlets only...

Page 23: ...tup is loaded automati cally Before switching on your CASY Instrument ensure that the control panel is connected and proper ly bolted to the measuring stand Removing the plug from the control panel wh...

Page 24: ...to the sampling chamber up to the limit stop so that the sample is shielded on all sides Selecting an appropriate setup see Chapter 5 3 or manually inputting the parameters see Chapter 5 4 Monitoring...

Page 25: ...re the samples the CASY ton used to flush the system and dilute the samples and also any CASY cups used are free of grease and particle im purities For routine preparation of the measuring samples use...

Page 26: ...s by holding the CLEAN key down and selecting 3 cycles 5 Perform a triple measurement using a measuring volume of 400 l and pressing START The error message Concentration too high may be displayed whi...

Page 27: ...ou tine Please always follow this procedure to shut down the system 1 Carry out steps 1 to 10 of the Weekly cleaning routine 2 Replace the two CASY cups containing distilled water with two empty CASY...

Page 28: ...d do not have to be stored in one of the setups 5 2 Menu Control MAIN MENU All settings of the CASY System are selected with the MENU key Pressing MENU displays the MAIN MENU MAIN MENU TT 2AB 9999 15...

Page 29: ...th the factory default set of parameters Pressing the NEXT key selects a setup You may select only those setups that have been logged and tagged with Pressing and holding down the NEXT key enables you...

Page 30: ...rhorizontalline containsallthemeasuringparameters These parameters must be defined before a measurement takes place They may not be retrospectively changed The main block below contains all evaluation...

Page 31: ...to move backwards Y axis The Auto setting automatically modifies the Y axis scale according to the distribution maxi mum The Y axis scale can also be set in predefined steps between 10 and 7500 This e...

Page 32: ...and how the aggregation correction of your count results should be performed Off Manual and Auto are the available settings Further information can be found on www cellcounting de In the Auto setting...

Page 33: ...vance with the Page Feed command on your printer When the size distribution is printed Graphic to On P Feed is ignored Print Mode The print mode can be set to either Manual or Auto When set to Auto th...

Page 34: ...so unused setups If you select a setup tagged with its stored set tings will be overwritten with your new settings If you select an unused setup it will appear tagged with and will be available for se...

Page 35: ...th this measuring capillary is activated Please note that previously displayed measuring data are lost when a capillary setting is changed Pressing CANCEL enables you to exit the screen without changi...

Page 36: ...N OR ENTER TO CONFIRM CHANGES All measuring results date and time and the used setup can be selected as Graph Para meters Each parameter has its own abbreviation consisting of up to three characters w...

Page 37: ...o revise your selection Deleting the currently active setup will also delete the current measuring data 5 8 MAIN MENU Service Menu The Service Menu contains the Dry Liquid System command you require t...

Page 38: ...fy parameters or create a new setup can be found in Chapter 6 5 6 1 Preparing Samples Fill a CASY cup with 10 ml filtered CASY ton Add an aliquot of the cell suspension to be examined normally between...

Page 39: ...ugh all the necessary steps After a short term the size distribution for your sample appears in the display Instructions for setting up the major applications can be found in the CASY TT Customer Trai...

Page 40: ...IME 10 15 AGG MANUAL NORM 7 60 40 00 m SN TT 2XX 9999 PVOL 4 249E 03 fl EVAL 12 80 40 00 m Counts 2911 ConcRng OK m ml 1 500e 02 Aggreg 1 157 Viable ml 1 684e 05 Viable 83 04 Total ml 2 028e 05 Debris...

Page 41: ...are taken into account in the calculation of all values indi cated in milliliters ml Counts Number of particles cells counted in the size range of the evaluation cursors ConcRng Indicates whether the...

Page 42: ...ystem undergoing the regular Weekly Cleaning Routine see Chapter 4 1 using CASY clean see Chapter 4 1 You must ensure that your CASY System is also kept clean between cleaning cycles Specifically that...

Page 43: ...luation cursor settings They are always determined for the entire physical measuring range of the measuring capillary being used approx 2 to 80 of the measuring pore diameter In the case of cell sampl...

Page 44: ...CONFIRM PRESS CLEAN TO FLUSH THE LIQUID SYSTEM Most blockages occurring during the measuring operation result from the temporary presence of very large particles e g aggregates tissue remnants in fro...

Page 45: ...able to see the air bubble If the air bubble rises to the fluid meniscus and disappears it will not affect subsequent measurements You can repeat the measurement immediately after confirming the erro...

Page 46: ...uring range and cursor positions being correctly adjusted It is particularly important for the reproducibility and comparability of the data that all samples to be compared are measured using identica...

Page 47: ...normalization cursor you must first perform a measurement on a culture with high viability Then kill an aliquot of your cells and measure the size distribution of these dead cells To finally set your...

Page 48: ...ted in the top right hand corner of the size distribution Pressing L Cur sor R Cursor and NEXT enables you to select a different cursor to move at any time The manual cursor setting mode can be termin...

Page 49: ...7 843e 04 Print Mode Manual The Comment and User Signature rows are intended for handwritten comments on the measuring results and a signature where appropriate The size distribution is inserted betwe...

Page 50: ...by a hyphen and a one or two figure number is output such as for example PR 7 In special cases a combination of up to 12 hexadecimal characters may be output which are arranged in 3 blocks separated...

Page 51: ...Y Instru ment 5 Check if the background decreases if no cleaning cycle is started but only a measurement with a new CASY cup containing freshly filtered CASY ton Repeat this step twice and record the...

Page 52: ...s with CASY clean are recommended to avoid prob lems with blocked capillaries see section Maintenance and Care If the message Measuring Timeout Capillary is possibly blocked PR 7 is displayed often or...

Page 53: ...avoid prob lems with air bubbles see section Maintenance and Care If the message Large Air Bubble detected is displayed often try the following procedure checking after each step if the problem is res...

Page 54: ...CASY cups Empty the two cups and put them back into their position Open the SERVICE MENU screen and start the Dry Liquid System command Start the Dry Liquid System command a second time Put the stora...

Page 55: ...e CL 1 is displayed during self test the instrument has lost its calibration par tially or completely wrong check sum In this case the calibration needs to be re loaded 1 Contact your OLS representati...

Page 56: ...tion Measure a sample with a clear cut well separated peak of viable cells as well as a viability 90 at least 5 times 2 000 10 000 cells per measurement Enter the average of the measured peak volume i...

Page 57: ...cell clusters may exist which occur with a probability of 1 1 000 compared to single cells the chance to see such a big cell cluster would only be about once in 10 measurements since you can see 100...

Page 58: ...k if the COM port settings in terms of the communication ports in the CASY excell Setup sheet fits to the COM port connecting the CASY Instrument with the computer using the serial cable 4 If neccessa...

Page 59: ...ement If Print is set to Manual the data are output by pressing PRINT on the control panel The data are exported in text format ASCII and the individual values are separated by CR LF To be able to acc...

Page 60: ...ange 0 Ok 1 Too High 422 Counts Upper Scaling Limit ml e 423 Aggregation Factor 424 Counts ml e within evaluation cursor range 425 Total Counts ml e within normalization cursor range 426 Viable Cells...

Page 61: ...1 T 160mA SI2 T 1 6A SI3 T 400mA SI4 T 2 5A SI5 T 400mA Installation category II according to IEC 61010 1 Volume measuring section and pressure system Calibrated opto electronically controlled volume...

Page 62: ...oring of the measuring pore Automatic flushing of the measuring pore Automatic detection and display of operating and system errors Integrated service functions for fast error diagnosis Fan less syste...

Page 63: ......

Page 64: ...research only Not for use in diagnostic procedures OMNI Life Science GmbH Co KG Karl Ferdinand Braun Stra e 2 28359 Bremen T 49 421 276 16 9 0 F 49 421 276 16 9 19 info ols bio de www ols bio de www...

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