15
Introduction
Aggregation Correction
The CASY Cell Counter and Analyzer offers a method to analyze even clumpy cell suspensions with
its integrated aggregation correction. By analyzing the size distribution, the CASY Cell Counter and
Analyzer determines the statistically most occurring cell size, the volume of the statistically “repre-
sentative single cell”. The typical single cell volume is displayed in the analysis protocol as the peak
volume. The total volume of all measured events in the size range of single cells plus aggregates is
divided by the volume of the representative single cell to calculate the total number of single cells.
Cell aggregates thereby are classified into their single cells, thus making it possible to determine
the single cell count including the number of single cells in aggregates. The CASY Cell Counter and
Analyzer represents this correction in the form of an aggregation factor. The mean aggregation fac-
tor is used to record the aggregation correction. This parameter also can be used as a further quality
control parameter for the characterization of cell cultures.
For more information on using aggregation correction and determining the aggregation factor,
please visit www.cellcounting.de
.
1.4 CASY TT
The CASY TT combines a high degree of ergonomics with functionality. At the push of a button, the
electronics:
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Control the measuring stand
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Record the measuring data
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Evaluate the measuring data via a multi-channel analyzer
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Calculate and display the measuring results on the CASY Control Panel
All measuring parameters can be set up with a minimum of steps. For each measuring capillary, up
to 20 settings can be permanently stored for various cell types or various applications and remain
available the next time the device is switched on.
To output the data, a printer (at least PCL 3) can be directly connected to the device. For more
convenient display, storage and further evaluations, the measurement data can also be exported to
Microsoft Excel through a fully automated process using the CASY excell Software which is delivered
together with each system. For more information on CASY excell see the installation instruction for
CASY excell or visit www.cellcounting.de
.
Aggregation Correction
