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CASY TT Operator‘s Guide
MAIN MENU – Edit Setup
%Calculation:
In addition to the
Off
setting (percentage calculation disabled), three other percentage calcu-
lation settings (%Via, %Cnt and %Vol) are available.
%Via:
This setting calculates the viability of a cell sample in percentage terms. It is
assumed that the evaluation cursor is set to the viable cell size range and the
normalization cursor covers the size range of all cells (dead + viable).
%Cnt:
This setting calculates the percentage counts in the size range of the evalua-
tion cursor in relation to the size range of the normalization cursor, which is set
to 100%.
%Vol:
This setting calculates the percentage biovolumes in the size range of the evalu-
ation cursor in relation to the size range of the normalization cursor, which is set
to 100%.
Debris:
This parameter can be either
On
or
Off
. It can be set to
On
only if para meter
%Via
is
selected from %Calculation. When set to
On
, all counts left of the left normalization cur-
sor are summed up and output as debris/ml taking into account measuring volume and
dilution factor.
Aggr.
Correct:
This parameter defines whether and how the
aggregation correction
of your count
results should be performed.
Off
,
Manual
and
Auto
are the available settings (Further
information can be found on www.cellcounting.de. In the
Auto
setting, the aggregation
factor of a sample is automatically calculated as the quotient of mean volume and peak
volume. The calculation is based on the following assumptions:
a) The evaluation cursors are set to the viable cell size range, including all aggregates.
b) The size distribution of the viable cells exhibits a clear maximum.
c) The measurement is supported by adequate statistical reliability
(>1000 count results in the viable cell range).
If your samples do not match Item b) and/or c), you can specify the volume of the
individual cells manually. To do this, you must select the manual setting and enter the
mean individual volume of your sample using para meter
P.Vol
(see below). This setting
is always recommended if the volume of your cells does not change significantly dur-
ing the course of an experiment. The aggregate correction facility is normally used in
conjunction with the
%Via
percentage calculation.
P.Vol:
This parameter defines the mean volume of individual cells in your samples for the
manual aggregation correction (see Aggr.Correct) in terms of femtolitres. The value is
entered in exponential format.
