58
OpenSPR 1-Channel Operation Manual V4.1.01
solution. This will pass the cleaning solution through the flow cell and fluidic lines that are
exposed to user samples.
5.
Once the bubble removal sequence with the 0.5% SDS is complete, rinse the injection port
with 3 mL of DI water.
6.
Rinse the injection port with 2 mL of 6 M urea. While the solution is still within the
OpenSPR, perform a bubble removal injection of this solution.
7.
Once the bubble removal sequence with the 6 M urea is complete, rinse the injection port
with 3 mL of DI water.
8.
Rinse the injection port with 2 mL of 0.2 M sodium bicarbonate. While the solution is still
within the OpenSPR, perform a bubble removal injection of this solution.
9.
Once the bubble removal sequence with the 0.2 M sodium bicarbonate is complete, rinse
the injection port with 3 mL of DI water.
10.
Rinse the injection port with 2 mL of 1% acetic acid. While the solution is still within the
OpenSPR, perform a bubble removal injection of this solution.
11.
Once the bubble removal sequence with the 1% acetic acid is complete, rinse the injection
port with 3 mL of DI water.
12.
Rinse the injection port with 2 mL of 0.5% sodium hypochlorite. While the solution is still
within the OpenSPR, perform a bubble removal injection of this solution.
13.
Once the bubble removal sequence with the 0.5% sodium hypochlorite is complete, rinse
the injection port with 3 mL of DI water.
14.
Rinse the injection port with at least another 3 mL of DI water to fully clear the lines.
