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OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.69 Selection of running buffer bottle to place the instrument into standby mode.
Note:
Standby mode will use consume approximately 8 mL of running buffer or distilled
water / 24 hrs. Prior to entering standby mode, ensure sufficient solutions are present in
the buffer bottles.
3.
Once the standby buffer is selected, the instrument will perform an initial rinsing
procedure. This will take approximately 21 minutes to complete. During this time, the
user must rinse out the injection port with at least 3 mL
4.
When the rinsing procedure is complete, the instrument will enter standby mode and the
software will remain on the home screen. The status of the instrument will be displayed,
and the instrument can be left unattended.
Important:
Do not shut down the software or the OpenSPR instrument while in standby
mode.
2.6.3
Instrument Shutdown
Prior to shutting down the instrument, it is important to thoroughly rinse and sterilize the tubing
to maintain proper performance of the OpenSPR.
The shutdown procedure will require the following supplies:
•
At least 20 mL of filtered deionized water
•
At least 20 mL of 80% isopropanol
•
Blank chip
•
2x syringes for rinsing the injection port with deionized water and 80% isopropanol
The full shutdown procedure will take approximately 90 minutes to complete and consists of 3
steps: a rinse with deionized water, sterilization with 80% isopropanol, followed by purging of
the pumps and tubing with air. It is recommended to empty the waste bottle prior to beginning
