8
OpenSPR 1-Channel Operation Manual V4.1.01
1
Gastight glass syringe (250 µL)
1
Training Kit
1
Declogging Kit
1
Screwdriver (for servicing)
1.1.3
Setting up the OpenSPR
1.
Remove the OpenSPR Instrument from the box, and place on a clean, level surface.
2.
Remove the Power Cable and USB Cable from the bottle tray of the instrument (right-
hand side) and remove all additional packaging tape present on the instrument.
3.
Remove the twist-ties from the tubes within the bottle tray.
4.
Unpack the glass bottles from the Accessory Box and remove all tape and stickers from
the lid and fittings.
5.
Locate the 250 mL glass bottle that does not contain the tube fitting (without the black
attachment on top). This will be your Waste Bottle (tip: it is good practice to label this
bottle accordingly). Find the 3 outlet tubes located in a bundle at the back of the bottle
tray. They will be labelled “Sample Waste”, “Outlet Waste”, and “Pump Waste”. Place all
3 tubes through the openings in the bottle cap, then place bottle at the back of the bottle
tray [Figure 1.1].
Figure 1.1 Waste bottle installed in the OpenSPR with 3 outlet tubes.
6.
Locate the 3 remaining 250 mL glass bottles. Fill one of the bottles with 250 mL of filtered
DI water (use a 0.2 µm filter) or MilliQ water, and label accordingly. The remaining 2
bottles will be used for your running buffers (leave these empty until use, but still proceed
with the setup).
