43
OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.53 Solid and dotted lines on the response graph marking the beginning and end of the injection period.
The user will not be able to perform other instrument functions during the injection
period.
6.
Only after the injection is complete and the baseline has settled should the user move
onto the next injection, repeating this procedure.
2.5.6
Ligand Immobilization Wizard
The OpenSPR software contains a built-in ligand immobilization wizard which walks the user
though the recommended procedures for immobilization of a ligand onto the various sensor
chemistries available.
The ligand immobilization wizard can be accessed by clicking the “Ligand” button in the wizard
section of the menu bar [Figure 2.54].
Figure 2.54 Button to access the ligand wizard in the menu bar.
Upon opening the wizard, the user will first be prompted to select the desired sensor chemistry
[Figure 2.55]. Each chemistry will have a unique built-in protocol of for the user to follow. The
protocol will walk the user through the various injections needed (surface clearing, surface
activation, etc) with built-in controls for the recommended flow rates and channel settings.
