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OpenSPR 1-Channel Operation Manual V4.1.01
2.4.2
Step 4: Bubble Removal and Prevention
It is important to remove and prevent any bubbles within the fluidic lines prior to starting your
test. Bubbles present in the system can cause interference with the sensor signal. The software
contains a semi-automated built-in procedure for removing bubbles. This procedure can be
accessed immediately following the load sensor process or can be started by selecting the
“Bubble Removal” button on the Sensor Setup Screen [
Figure 2.35]. Details for the procedure are
found below.
Figure 2.35 Button to commence the bubble removal process.
1.
To remove bubbles, a solution of 80% isopropanol will be used. The software will prompt
you to load this solution into the injection port
–
at least 150 µL volume is recommended
[Figure 2.36]. The solution should be introduced into the OpenSPR using a designated
syringe with a blunt-end injection tip.
Figure 2.36 Bubble Removal Setup prompt.
2.
After the 80% isopropanol is loaded into the instrument, click “Next” to begin the
automated bubble removal procedure. This procedure will take approximately 2 minutes
to complete. The time remaining will be displayed with a progress bar [Figure 2.37].
Figure 2.37 Bubble removal progress dialogue.
During this period, the 80% isopropanol will be passed through the flow cell. The
decreased surface tension of the isopropanol solution will push out any pre-existing
bubbles towards the outlet waste.
