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OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.59 Ligand evaluation stage.
Once the ligand immobilization is complete, the user will return to the main screen. It is
recommended to zero the baseline at this time (see Section 2.5.7) prior to beginning analyte
injection analysis.
2.5.7
Graph Settings
The user has the flexibility to customize the view of the response graph in real time throughout
the experiment. The primary graph controls are accessed in the menu bar of the main screen
[Figure 2.60]. In the menu, the user can zero the graph, as well as open advanced settings to
manually adjust the axes scale.
Figure 2.60 Graph controls in menu bar.
Zeroing the Graph
Clicking “Zero” in the graph menu
[Figure 2.61] will clear all the data points from the graph and
will normalize the response tracking to zero on the y-axis. All the previously collected data is still
saved to the data file but is only no longer being displayed on the graph.
Zeroing the graph is recommended at any stage in the experiment where a new baseline is
established, and the user wants to make a relative comparison for subsequent injections. An
example of this scenario is after the ligand immobilization step, and before injection of analyte
for analysis.