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OpenSPR 1-Channel Operation Manual V4.1.01
long as it stays on the plunger side and is not introduced into the OpenSPR with your sample
[Figure 2.48].
Figure 2.48 Example of loading sample into disposable plastic syringe with air gap.
Glass syringes (SYR-G) are 250 µL in volume and come installed with a gastight blunt-end injection
tip which does not contain a dead volume (replacement needles are also available, RN-G-6).
These syringes are recommended to be used for performing injection of biological samples
(ligand and analyte), where precise volume control at 150 µL is desired. If using the same glass
syringe for multiple samples, it is recommended to rinse the syringe thoroughly with running
buffer between samples.
Volume for Sample Injection:
The amount of sample volume required for an injection is dependent on the size of sample loop
installed in the OpenSPR. The minimum sample volume required is the volume of the sample
loop + 50 µL. Excess volume in addition to that of the sample loop is required to ensure a uniform
sample concentration profile and prevent air from remaining within the sample loop.
The standard size of sample loop provided is 100 µL in volume.
The minimum injection volume
for a 100 µL sample loop is 150 µL.
For general solutions such as 80% isopropanol, regeneration buffers, etc it is recommended to
use excess volume to prevent user error in the injection process when possible.
Injection Process:
1.
Rinse the injection port and sample loop with running buffer to flush any previous
sample/solution from the sample loop fluidic lines.
To do this, fill a syringe (designated for the running buffer) with at least 0.5 mL of the
running buffer*. Insert the blunt tipped syringe fully into the injection port making sure it
is seated, then slowly depress the plunger allowing all the running buffer to be passed
