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OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.57 Example dialogue box containing injection instructions and inject button.
When the user reaches the Ligand Immobilization step, they will be prompted to enter their
ligand details, including the name and concentration [Figure 2.58]. The user can also set the
contact time manually if desired. Note that the contact time will be dependent on the sample
loop volume installed in the instrument. For a 100 µL sample loop, for most applications a 300 s
contact time is recommended, but the time can be adjusted from 1200
–
30 s.
Figure 2.58 Ligand immobilization injection stage.
Upon performing the ligand injection, the wizard will automatically move forward onto the
evaluation stage [Figure 2.59]. The software will automatically evaluate the amount of ligand
immobilization obtained and will compare it against the minimum immobilization target level
determined at the start of the wizard. If enough ligand immobilization was obtained, the user can
move onto the next step. If not, it is recommended that the user repeats another injection of the
ligand. If the ligand immobilization level achieved is significantly lower than the target, the user
should attempt to increase the concentration of the ligand used.
