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OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.9 Option to label your running buffers.
The completion of this step is not required for running a test but is recommended for your
experimental records.
2.3.2
Step 1: Fluidic Priming
Priming of the OpenSPR will fill the pumps and tubing with the running buffer to be used for the
test.
It is necessary to perform priming in the below scenarios:
1)
Initial use of the instrument from purchase
2)
If the instrument is being used for the first time after a full shutdown
3)
If you would like to use a new running buffer for your test
4)
If you are going to be starting with a running buffer in an alternate bottle position
If you are running a test with the running buffer already present in the system, using the same
bottle position and if no air has been introduced into the inlet tubing, then you do not need to
perform the priming procedure. In this scenario, you can leave the fluidic priming as incomplete
to move forwards with your test setup.
To prime the instrument with a new running buffer,
click the “
Prime Process
Start” button [
Figure
2.10] and follow the on-screen prompts.
