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OpenSPR 1-Channel Operation Manual V4.1.01
3.
Once the bubble removal procedure is complete, you will be prompted to perform a visual
inspection of the flow cell [Figure 2.38]. You will want to look for the presence of any
bubbles within the full fluidic path
–
it is easiest to look for the outline of an air-liquid
interface [Figure 2.39]
. If any bubbles are still present, click “Repeat”, which will bring you
back to the initial step, where you will need to load more isopropanol into the injection
port. Repeat this process until all bubbles are removed.
Figure 2.38 Bubble injection complete prompt.
Figure 2.39 Example of bubble present in flow cell channel.
4.
Once the flow cell channels are clear, click “Finish” to end the process. The bubble
removal status will be updated accordingly on the main screen.
5.
Prior to moving forward with your experiment, rinse the injection port with at least 0.5
mL of running buffer.
