9
OpenSPR 1-Channel Operation Manual V4.1.01
7.
The 3 inlet tubes will be inserted into Buffer Bottles 1, 2 and DI Water, respectively, from
front to back of the instrument. The tubes are labelled accordingly. Keep the bottles
outside of the instrument while inserting the tubes.
8.
To insert the tubes, loosen the black fitting by turning it counter-clockwise [Figure 1.2-
Left]. Note that there is a translucent ferrule beneath the black fitting. Once the fitting is
loosened, feed the tube through the fitting into the bottle, until the tube touches the
bottom [Figure 1.2
–
Right]. When the tube is in the final position, gently tighten the fitting
clockwise until the tube is secured into place (do not over tighten).
Figure 1.2 Left: Loosen the fitting by turning it counter-clockwise before inserting the tube. Right: Inlet tubes properly installed
with the end of the tubes touching the bottom of the bottles.
Note: if buffer bottles 1 and 2 are not going to be used immediately, it is recommended to store
the tubes within the empty bottles to prevent dust from entering the fluidic system.
9.
Insert the bottles into the tray by tilting the bottle such that the base of the bottle fits
into the tray first, followed by the top [Figure 1.3].
Figure 1.3 Left: Insert the bottles into the tray base-first and tilted. Right: Proper positioning of all bottles installed in the
OpenSPR.
